Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor

Ying Zhou, Xiaoyu Zhu, Chong Zhang, Wai Siong Chai, Kit Wayne Chew, Anning Du, Pau Loke Show

Research output: Journal PublicationArticlepeer-review

28 Citations (Scopus)


In this current study, whey protein isolate (WPI)/pectin biowaste composite film properties was investigated with addition of Streptomyces coelicolor-derived small laccase (SLAC). It was found that different SLAC concentrations has insignificant effect on the film color while best film transparency obtained with 4 U/100 mL SLAC. Optimum mechanical properties were obtained with 6 U/100 mL SLAC indicating best crosslinking effect on the WPI-pectin film. Lowest moisture content (MC), water vapor transmittance (WVP) and oxygen transmittance (O2P) of the film obtained with 6 U/100 mL SLAC, while 4 U/100 mL SLAC resulted in lowest carbon dioxide transmittance (CO2P). Maximum increase in phase transition temperature and absorption peak shift according to DSC and FTIR results, respectively, indicated largest improvement in heat resistance and chemical bond strength of the film with 6 U/100 mL SLAC addition. The XRD results showed that the addition of SLAC was insignificant towards the film crystallinity. The work presents the development of new degradable composite film that can be derived from biowaste compounds for utilization in food processing application.

Original languageEnglish
Article number100999
JournalEnvironmental Technology and Innovation
Publication statusPublished - Aug 2020
Externally publishedYes


  • Composite film
  • Pectin
  • Small laccase
  • Streptomyces coelicolor
  • Whey protein isolate

ASJC Scopus subject areas

  • General Environmental Science
  • Soil Science
  • Plant Science


Dive into the research topics of 'Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor'. Together they form a unique fingerprint.

Cite this