Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor

Ying Zhou; Xiaoyu Zhu; Chong Zhang; Wai Siong Chai; Kit Wayne Chew; Anning Du; Pau Loke Show

doi:10.1016/j.eti.2020.100999

Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor

Ying Zhou, Xiaoyu Zhu, Chong Zhang, Wai Siong Chai, Kit Wayne Chew, Anning Du, Pau Loke Show

Research output: Journal Publication › Article › peer-review

29 Citations (Scopus)

Abstract

In this current study, whey protein isolate (WPI)/pectin biowaste composite film properties was investigated with addition of Streptomyces coelicolor-derived small laccase (SLAC). It was found that different SLAC concentrations has insignificant effect on the film color while best film transparency obtained with 4 U/100 mL SLAC. Optimum mechanical properties were obtained with 6 U/100 mL SLAC indicating best crosslinking effect on the WPI-pectin film. Lowest moisture content (MC), water vapor transmittance (WVP) and oxygen transmittance (O₂P) of the film obtained with 6 U/100 mL SLAC, while 4 U/100 mL SLAC resulted in lowest carbon dioxide transmittance (CO₂P). Maximum increase in phase transition temperature and absorption peak shift according to DSC and FTIR results, respectively, indicated largest improvement in heat resistance and chemical bond strength of the film with 6 U/100 mL SLAC addition. The XRD results showed that the addition of SLAC was insignificant towards the film crystallinity. The work presents the development of new degradable composite film that can be derived from biowaste compounds for utilization in food processing application.

Original language	English
Article number	100999
Journal	Environmental Technology and Innovation
Volume	19
DOIs	https://doi.org/10.1016/j.eti.2020.100999
Publication status	Published - Aug 2020
Externally published	Yes

Keywords

Composite film
Pectin
Small laccase
Streptomyces coelicolor
Whey protein isolate

ASJC Scopus subject areas

General Environmental Science
Soil Science
Plant Science

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10.1016/j.eti.2020.100999

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title = "Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor",

abstract = "In this current study, whey protein isolate (WPI)/pectin biowaste composite film properties was investigated with addition of Streptomyces coelicolor-derived small laccase (SLAC). It was found that different SLAC concentrations has insignificant effect on the film color while best film transparency obtained with 4 U/100 mL SLAC. Optimum mechanical properties were obtained with 6 U/100 mL SLAC indicating best crosslinking effect on the WPI-pectin film. Lowest moisture content (MC), water vapor transmittance (WVP) and oxygen transmittance (O2P) of the film obtained with 6 U/100 mL SLAC, while 4 U/100 mL SLAC resulted in lowest carbon dioxide transmittance (CO2P). Maximum increase in phase transition temperature and absorption peak shift according to DSC and FTIR results, respectively, indicated largest improvement in heat resistance and chemical bond strength of the film with 6 U/100 mL SLAC addition. The XRD results showed that the addition of SLAC was insignificant towards the film crystallinity. The work presents the development of new degradable composite film that can be derived from biowaste compounds for utilization in food processing application.",

keywords = "Composite film, Pectin, Small laccase, Streptomyces coelicolor, Whey protein isolate",

author = "Ying Zhou and Xiaoyu Zhu and Chong Zhang and Chai, {Wai Siong} and Chew, {Kit Wayne} and Anning Du and Show, {Pau Loke}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = aug,

doi = "10.1016/j.eti.2020.100999",

language = "English",

volume = "19",

journal = "Environmental Technology and Innovation",

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TY - JOUR

T1 - Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor

AU - Zhou, Ying

AU - Zhu, Xiaoyu

AU - Zhang, Chong

AU - Chai, Wai Siong

AU - Chew, Kit Wayne

AU - Du, Anning

AU - Show, Pau Loke

PY - 2020/8

Y1 - 2020/8

N2 - In this current study, whey protein isolate (WPI)/pectin biowaste composite film properties was investigated with addition of Streptomyces coelicolor-derived small laccase (SLAC). It was found that different SLAC concentrations has insignificant effect on the film color while best film transparency obtained with 4 U/100 mL SLAC. Optimum mechanical properties were obtained with 6 U/100 mL SLAC indicating best crosslinking effect on the WPI-pectin film. Lowest moisture content (MC), water vapor transmittance (WVP) and oxygen transmittance (O2P) of the film obtained with 6 U/100 mL SLAC, while 4 U/100 mL SLAC resulted in lowest carbon dioxide transmittance (CO2P). Maximum increase in phase transition temperature and absorption peak shift according to DSC and FTIR results, respectively, indicated largest improvement in heat resistance and chemical bond strength of the film with 6 U/100 mL SLAC addition. The XRD results showed that the addition of SLAC was insignificant towards the film crystallinity. The work presents the development of new degradable composite film that can be derived from biowaste compounds for utilization in food processing application.

AB - In this current study, whey protein isolate (WPI)/pectin biowaste composite film properties was investigated with addition of Streptomyces coelicolor-derived small laccase (SLAC). It was found that different SLAC concentrations has insignificant effect on the film color while best film transparency obtained with 4 U/100 mL SLAC. Optimum mechanical properties were obtained with 6 U/100 mL SLAC indicating best crosslinking effect on the WPI-pectin film. Lowest moisture content (MC), water vapor transmittance (WVP) and oxygen transmittance (O2P) of the film obtained with 6 U/100 mL SLAC, while 4 U/100 mL SLAC resulted in lowest carbon dioxide transmittance (CO2P). Maximum increase in phase transition temperature and absorption peak shift according to DSC and FTIR results, respectively, indicated largest improvement in heat resistance and chemical bond strength of the film with 6 U/100 mL SLAC addition. The XRD results showed that the addition of SLAC was insignificant towards the film crystallinity. The work presents the development of new degradable composite film that can be derived from biowaste compounds for utilization in food processing application.

KW - Composite film

KW - Pectin

KW - Small laccase

KW - Streptomyces coelicolor

KW - Whey protein isolate

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U2 - 10.1016/j.eti.2020.100999

DO - 10.1016/j.eti.2020.100999

M3 - Article

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SN - 2352-1864

VL - 19

JO - Environmental Technology and Innovation

JF - Environmental Technology and Innovation

M1 - 100999

ER -

Characterization of whey protein isolate and pectin composite film catalyzed by small laccase from Streptomyces coelicolor

Abstract

Keywords

ASJC Scopus subject areas

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