TY - JOUR
T1 - Simultaneous detection of fumonisin B 1 and ochratoxin A using dual-color, time-resolved luminescent nanoparticles (NaYF 4 : Ce, Tb and NH 2 -Eu/DPA@SiO 2 ) as labels
AU - Niazi, Sobia
AU - Khan, Imran Mahmood
AU - Yan, Lv
AU - Khan, Muhammad Issa
AU - Mohsin, Ali
AU - Duan, Nuo
AU - Wu, Shijia
AU - Wang, Zhouping
N1 - Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B 1 (FB 1 ) was developed using multi-color, Ln 3+ -doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF 4 : Ce, Tb and NH 2 -Eu/DPA@SiO 2 NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe 3 O 4 magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb 3+ and Eu 3+ , respectively, were used to measure FB 1 (Y = 19,177.1 + (− 12,054.4)x, R 2 = 0.9917) and OTA (Y = 4138.8 + (− 11,182.6)x, R 2 = 0.9924), respectively. The limits of detection (LODs) for FB 1 and OTA were 0.019 pg mL −1 and 0.015 pg mL −1 , respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB 1 while detection range varied from 0.0001–0.5 ng mL −1 . This aptasensor was effectively applied to quantity FB 1 and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields. [Figure not available: see fulltext.].
AB - A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B 1 (FB 1 ) was developed using multi-color, Ln 3+ -doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF 4 : Ce, Tb and NH 2 -Eu/DPA@SiO 2 NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe 3 O 4 magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb 3+ and Eu 3+ , respectively, were used to measure FB 1 (Y = 19,177.1 + (− 12,054.4)x, R 2 = 0.9917) and OTA (Y = 4138.8 + (− 11,182.6)x, R 2 = 0.9924), respectively. The limits of detection (LODs) for FB 1 and OTA were 0.019 pg mL −1 and 0.015 pg mL −1 , respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB 1 while detection range varied from 0.0001–0.5 ng mL −1 . This aptasensor was effectively applied to quantity FB 1 and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields. [Figure not available: see fulltext.].
KW - Aptamer
KW - Mycotoxins
KW - Simultaneous detection
KW - Time-resolved fluorescence nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85061318307&partnerID=8YFLogxK
U2 - 10.1007/s00216-019-01580-0
DO - 10.1007/s00216-019-01580-0
M3 - Article
C2 - 30739197
AN - SCOPUS:85061318307
SN - 1618-2642
VL - 411
SP - 1453
EP - 1465
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 7
ER -