Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures

Nikhil Swaraj  Arige; Gayatri  Dangeti; Kandi Sridhar; Minaxi Sharma; Baskaran Stephen Inbaraj; Praveen Kumar  Dikkala; Viharika  Kondru

doi:10.1007/978-1-0716-4490-4_7

Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures

Nikhil Swaraj Arige, Gayatri Dangeti, Kandi Sridhar, Minaxi Sharma, Baskaran Stephen Inbaraj, Praveen Kumar Dikkala , Viharika Kondru

CBI Life Science and Healthcare Centre

Research output: Chapter in Book/Conference proceeding › Book Chapter › peer-review

Abstract

This chapter presents a technical protocol for cellulase enzyme production via solid-state fermentation (SSF) using groundnut (Arachis hypogea L.) shells, a lignocellulosic agro-waste, as a substrate. Groundnut shells, containing 65.7% cellulose and 59.0% crude fiber, are pretreated using alkaline hydrolysis to disrupt lignocellulosic bonds and facilitate microbial access. Aspergillus niger, known for its cellulolytic capabilities, is used as a microbial agent. The chapter describes the preparation of spore suspensions, substrate sterilization, and inoculation processes to ensure optimal conditions for fungal growth. SSF is conducted at 30 °C under controlled moisture and humidity conditions, enhancing enzyme–substrate interaction. Cellulase extraction involves buffer-mediated recovery, followed by centrifugation and filtration to isolate crude enzyme. Cellulase activity is quantified using a filter paper assay (FPA), with reducing sugars measured spectrophotometrically via the dinitrosalicylic acid (DNS) method. This methodology maximizes cellulase yield, aligning with sustainable bioresource valorization within the circular bioeconomy framework.

Original language	English
Title of host publication	Fruit and Vegetable Waste Valorization
Editors	Kandi Sridhar, Venkata Seshamamba Burla, Minaxi Sharma, Baskaran Stephen Inbaraj
Publisher	Springer Nature
Chapter	7
ISBN (Electronic)	97810716449047
ISBN (Print)	9781071644898
DOIs	https://doi.org/10.1007/978-1-0716-4490-4_7
Publication status	Published - 26 Apr 2025

Access to Document

10.1007/978-1-0716-4490-4_7

Cite this

Arige, N. S., Dangeti, G., Sridhar, K., Sharma, M., Inbaraj, B. S., Dikkala , P. K., & Kondru , V. (2025). Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures. In K. Sridhar, V. S. Burla, M. Sharma, & B. S. Inbaraj (Eds.), Fruit and Vegetable Waste Valorization Springer Nature. https://doi.org/10.1007/978-1-0716-4490-4_7

@inbook{e3095c8d8a6545e8bbfbb1e5f7c624a9,

title = "Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures",

abstract = "This chapter presents a technical protocol for cellulase enzyme production via solid-state fermentation (SSF) using groundnut (Arachis hypogea L.) shells, a lignocellulosic agro-waste, as a substrate. Groundnut shells, containing 65.7% cellulose and 59.0% crude fiber, are pretreated using alkaline hydrolysis to disrupt lignocellulosic bonds and facilitate microbial access. Aspergillus niger, known for its cellulolytic capabilities, is used as a microbial agent. The chapter describes the preparation of spore suspensions, substrate sterilization, and inoculation processes to ensure optimal conditions for fungal growth. SSF is conducted at 30 °C under controlled moisture and humidity conditions, enhancing enzyme–substrate interaction. Cellulase extraction involves buffer-mediated recovery, followed by centrifugation and filtration to isolate crude enzyme. Cellulase activity is quantified using a filter paper assay (FPA), with reducing sugars measured spectrophotometrically via the dinitrosalicylic acid (DNS) method. This methodology maximizes cellulase yield, aligning with sustainable bioresource valorization within the circular bioeconomy framework.",

author = "Arige, {Nikhil Swaraj} and Gayatri Dangeti and Kandi Sridhar and Minaxi Sharma and Inbaraj, {Baskaran Stephen} and Dikkala, {Praveen Kumar} and Viharika Kondru",

year = "2025",

month = apr,

day = "26",

doi = "10.1007/978-1-0716-4490-4_7",

language = "English",

isbn = "9781071644898",

editor = "Kandi Sridhar and Burla, {Venkata Seshamamba } and Minaxi Sharma and Inbaraj, {Baskaran Stephen}",

booktitle = "Fruit and Vegetable Waste Valorization",

publisher = "Springer Nature",

address = "United States",

}

Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures. / Arige, Nikhil Swaraj; Dangeti, Gayatri; Sridhar, Kandi et al.
Fruit and Vegetable Waste Valorization. ed. / Kandi Sridhar; Venkata Seshamamba Burla; Minaxi Sharma; Baskaran Stephen Inbaraj. Springer Nature, 2025.

Research output: Chapter in Book/Conference proceeding › Book Chapter › peer-review

TY - CHAP

T1 - Cellulase Enzyme Production Through Solid State Fermentation of Peanut Shells Using Microbial Cultures

AU - Arige, Nikhil Swaraj

AU - Dangeti, Gayatri

AU - Sridhar, Kandi

AU - Sharma, Minaxi

AU - Inbaraj, Baskaran Stephen

AU - Dikkala , Praveen Kumar

AU - Kondru , Viharika

PY - 2025/4/26

Y1 - 2025/4/26

N2 - This chapter presents a technical protocol for cellulase enzyme production via solid-state fermentation (SSF) using groundnut (Arachis hypogea L.) shells, a lignocellulosic agro-waste, as a substrate. Groundnut shells, containing 65.7% cellulose and 59.0% crude fiber, are pretreated using alkaline hydrolysis to disrupt lignocellulosic bonds and facilitate microbial access. Aspergillus niger, known for its cellulolytic capabilities, is used as a microbial agent. The chapter describes the preparation of spore suspensions, substrate sterilization, and inoculation processes to ensure optimal conditions for fungal growth. SSF is conducted at 30 °C under controlled moisture and humidity conditions, enhancing enzyme–substrate interaction. Cellulase extraction involves buffer-mediated recovery, followed by centrifugation and filtration to isolate crude enzyme. Cellulase activity is quantified using a filter paper assay (FPA), with reducing sugars measured spectrophotometrically via the dinitrosalicylic acid (DNS) method. This methodology maximizes cellulase yield, aligning with sustainable bioresource valorization within the circular bioeconomy framework.

AB - This chapter presents a technical protocol for cellulase enzyme production via solid-state fermentation (SSF) using groundnut (Arachis hypogea L.) shells, a lignocellulosic agro-waste, as a substrate. Groundnut shells, containing 65.7% cellulose and 59.0% crude fiber, are pretreated using alkaline hydrolysis to disrupt lignocellulosic bonds and facilitate microbial access. Aspergillus niger, known for its cellulolytic capabilities, is used as a microbial agent. The chapter describes the preparation of spore suspensions, substrate sterilization, and inoculation processes to ensure optimal conditions for fungal growth. SSF is conducted at 30 °C under controlled moisture and humidity conditions, enhancing enzyme–substrate interaction. Cellulase extraction involves buffer-mediated recovery, followed by centrifugation and filtration to isolate crude enzyme. Cellulase activity is quantified using a filter paper assay (FPA), with reducing sugars measured spectrophotometrically via the dinitrosalicylic acid (DNS) method. This methodology maximizes cellulase yield, aligning with sustainable bioresource valorization within the circular bioeconomy framework.

U2 - 10.1007/978-1-0716-4490-4_7

DO - 10.1007/978-1-0716-4490-4_7

M3 - Book Chapter

SN - 9781071644898

BT - Fruit and Vegetable Waste Valorization

A2 - Sridhar, Kandi

A2 - Burla, Venkata Seshamamba

A2 - Sharma, Minaxi

A2 - Inbaraj, Baskaran Stephen

PB - Springer Nature

ER -