Removal of cationic dye waste by nanofiber membrane immobilized with waste proteins

Dinh Thi My Huong, Wai Siong Chai, Pau Loke Show, Yu Ling Lin, Chen Yaw Chiu, Shen Long Tsai, Yu Kaung Chang

Research output: Journal PublicationArticlepeer-review

46 Citations (Scopus)


Water pollution caused by dyes has been a serious problem affecting human health and environment. The surface of polyacrylonitrile (PAN) nanofiber membranes was modified by mild hydrolysis and coupled with bovine serum albumin (BSA) obtained from the laboratory wastes, resulting in the synthesis of P-COOH and P-COOH-BSA nanofibers. The nanofibers with specific functional groups may enhance their potential applications toward the removal of ionic dyes in wastewater. Toluidine blue O (TBO) was applied as an example of cationic dye to evaluate the removal efficiency of P-COOH-BSA nanofiber. Results showed that the equilibrium dissociation constant and maximum removal capacity were 0.48 mg/mL and 434.78 mg/g, respectively, at pH 12, where the TBO removal can be explained based on Langmuir isotherm and pseudo-second-order model. Desorption studies have shown that TBO adsorbed on P-COOH-BSA protein membrane can be completely eluted with either 1 M NaCl or 50% glycerol. The results of repeated studies indicated that after five consecutive adsorption/desorption cycles, the removal efficiency of TBO can be maintained at ~97%. P-COOH-BSA has shown to be promising adsorbent in TBO dye removal from dye wastewater.

Original languageEnglish
Pages (from-to)3873-3884
Number of pages12
JournalInternational Journal of Biological Macromolecules
Publication statusPublished - 1 Dec 2020
Externally publishedYes


  • BSA
  • Dye removal
  • Nanofiber membrane

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology


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