Optimization of hydrolysis-acidogenesis phase of swine manure for biogas production using two-stage anaerobic fermentation

Chiu Yue Lin, Wai Siong Chai, Chyi How Lay, Chin Chao Chen, Chun Yi Lee, Pau Loke Show

Research output: Journal PublicationArticlepeer-review

63 Citations (Scopus)


The traditional pig manure wastewater treatment in Taiwan has been low in methane production efficiency due to unstable influent concentration, wastewater volume, and quality. Two-stage anaerobic systems, in contrast, have the advantage of buffering the organic loading rate in the first stage (hydrolysis-acidogenesis phase), allowing a more constant feeding rate to the second stage (methanogenesis phase). Response surface methodology was applied to optimize the operational period (0.5–2.0 d) and initial operational pH (4–10) for hydrolysis and acidogenesis of the swine manure (total solid 5.3%) at 35C in batch operation mode. A methanogenesis verification experiment with the optimal condition of operational period 1.5 d and pH 6.5 using batch operation resulted in peak volatile acid production 7 g COD/L, methane production rate (MPR) 0.3 L-CH4/L-d, and methane yield (MY) 92 mL-CH4/g-CODre (chemical oxygen demand removed). Moreover, a two-stage system including a hydrolysis-acidogenesis reactor with the optimal operating condition and a methanogenesis reactor provided an average MPR 163 mL/L-d and MY 38 mL/g volatile solids, which values are 60% higher than those of a single-stage system; both systems have similar dominant methane-producing species of Firmicutes and Bacteroidetes with each having around 30%– 40%. The advantages of a two-stage anaerobic fermentation system in treating swine manure for biogas production are obvious.

Original languageEnglish
Article number1324
Issue number8
Publication statusPublished - Aug 2021
Externally publishedYes


  • Biomethane
  • Operational time
  • Response surface methodology (RSM)
  • Swine manure
  • Two-stage anaerobic digestion

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Process Chemistry and Technology


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