Melamine-based covalent organic polymers (Mcops) as lipase nanocarrier for recyclable esters hydrolysis and transesterification

Zhihao Wang, Ying Chen, Jiahe Zhao, Guoliang Gao, Worawan Panpipat, Ling Zhi Cheong, Cai Shen

Research output: Journal PublicationArticlepeer-review


Present study has successfully synthesized melamine-based covalent organic polymers (MCOPs) and applied it as lipase carrier for recyclable esters hydrolysis and transesterification. The synthesized MCOPs are composed of dense nanosheet structures having a thickness of 3.5 nm. Three immobilization methods namely physical adsorption, cross-linking and carrier activation were employed to prepare the MCOPs-immobilized CRL. Cross-linked MCOPs-immobilized CRL (41.30 mg protein/g MCOPs) and carrier activated MCOPs-immobilized CRL (33.20 mg protein/g MCOPs) had higher enzyme loading as compared to physical absorb MCOPs-immobilized CRL (29.30 mg protein/g MCOPs). Nevertheless, physical absorb MCOPs-immobilized CRL demonstrated the highest esters hydrolysis (49.85 U) and transesterification (1.04 U) activities. Despite having the highest enzymatic activity, physical absorb MCOPs-immobilized CRL were not able to maintain its catalytic stability with more than 30% decreased in enzymatic activity during consecutive hydrolysis and transesterification activities. Meanwhile, cross-linked MCOPs-immobilized CRL demonstrated highest catalytic stability with highest enzymatic activities at the end of consecutive reactions. All the MCOPs-immobilized CRL can be easily recovered and reused through centrifugation with more than 85% of recovery rate.

Original languageEnglish
Pages (from-to)627-634
Number of pages8
JournalJournal of Oleo Science
Issue number6
Publication statusPublished - 2020
Externally publishedYes


  • Candida rugosa lipase
  • Hydrolysis
  • Immobilization
  • Melamine-based covalent organic polymers
  • Transesterification

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


Dive into the research topics of 'Melamine-based covalent organic polymers (Mcops) as lipase nanocarrier for recyclable esters hydrolysis and transesterification'. Together they form a unique fingerprint.

Cite this