Cell clumping quantification and automatic area classification in peripheral blood smear images

Wei Xiong, S. H. Ong, Christina Kang, Joo Hwee Lim, Jiang Liu, Daniel Racoceanu, Kelvin Foong

Research output: Chapter in Book/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

Cell enumeration in peripheral blood smears and cell are widely applied in biological and pathological practice. Not every area in the smear is appropriate for enumeration due to severe cell clumping or sparseness arising from smear preparation. The automatic selection of good areas for cell enumeration can reduce manual labor and provide objective and consistent results. However, this has been infrequently studied and it is often difficult to count the exact number of cells in the clumps. To select good areas, we do not have to do this. Instead, we measure the goodness of such areas in terms of the degree of cell spread and the degree of clumping. The later is defined based on the distances and linking strengths of local voting peaks generated in the accumulator space after multi-scale circular Hough transforms. Support vector machines are then applied to classify the image areas into good or non-good classes. We have validated our method over 4500 testing cell images and achieved 89% sensitivity and 87% specificity.

Original languageEnglish
Title of host publicationProceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
DOIs
Publication statusPublished - 2009
Externally publishedYes
Event2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009 - Tianjin, China
Duration: 17 Oct 200919 Oct 2009

Publication series

NameProceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009

Conference

Conference2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
Country/TerritoryChina
CityTianjin
Period17/10/0919/10/09

Keywords

  • Automatic area classification
  • Cell clumping quantification
  • Cell enumeration
  • Hough transform
  • Multiscale
  • Peripheral blood smear images

ASJC Scopus subject areas

  • Biomedical Engineering
  • Health Informatics
  • Health Information Management

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