Carbon paste mediated, amperometric, thin film biosensors for fructose monitoring in honey

A. S. Bassi, E. Lee, J. X. Zhu

Research output: Journal PublicationArticlepeer-review

30 Citations (Scopus)

Abstract

A biosensor for the determination of fructose was constructed by the immobilization of the enzyme fructose dehydrogenase (FDH) behind a thin non-conducting electropolymer film of 1,3 phenylene diamine-resorcinol. Two different types of electrochemical mediators, soluble hexacyanoferrate (III) (FeCN) and the water insoluble tetracyanoquinodimethane (TCNQ) were applied as redox mediators for the amperometric measurement of fructose. Optimization of the biosensor was done with respect to operating conditions by varying the operating parameters such as pH, temperature, mediator concentration, and enzyme loading. The optimized biosensor was found to have a minimum detection limit of 10 μM with a linear range upto 1.0 mM. The performance of the two mediators was also compared with respect to stability. A simple, lumped parameter mathematical model was proposed to describe the steady state response of the biosensor and experimentally verified with the constructed biosensors.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalFood Research International
Volume31
Issue number2
DOIs
Publication statusPublished - Mar 1998
Externally publishedYes

Keywords

  • Biosensor
  • Fructose
  • Fructose dehydrogenase (FDH)
  • Hexacyanoferrate (III)
  • Mediated,
  • Tetracyanoquinodimethane (TCNQ)

ASJC Scopus subject areas

  • Food Science

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