The bradykinin B1 receptor has been considered as a receptor induced by tissue injury and inflammation mainly in the peripheral tissues. In the present study, we have investigated whether there is a basal expression in the spinal cord by both reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining methods. Southern blotting of the DNA reverse-transcribed from human and rat spinal cord mRNA and amplified by polymerase chain reaction (PCR) showed a substantial basal B1 receptor expression in both human and rat spinal cord. Immunohistochemical staining demonstrated B1-positive neurons in the spinal cord dorsal horn, suggesting that the B1 receptor is constitutively expressed by spinal neurons.
- B receptor
- Dorsal horn
- Polymerase chain reaction (PCR)
ASJC Scopus subject areas
- General Neuroscience