TY - JOUR
T1 - Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil
AU - Lee, Learn Han
AU - Azman, Adzzie Shazleen
AU - Zainal, Nurullhudda
AU - Yin, Wai Fong
AU - Ab Mutalib, Nurul Syakima
AU - Chan, Kok Gan
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2015
Y1 - 2015
N2 - Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0%), Sinomonas albida LC13T(97.9%) and Sinomonas soli CW 59T (97.8%), and lower (<97.6%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27%) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0%) of the cell membrane were anteiso-C15: 0(39.4%), C18: 1ω7c (17.7%), anteiso-C17: 0(17.2%) and iso-C16: 0(11.4%). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).
AB - Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0%), Sinomonas albida LC13T(97.9%) and Sinomonas soli CW 59T (97.8%), and lower (<97.6%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27%) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0%) of the cell membrane were anteiso-C15: 0(39.4%), C18: 1ω7c (17.7%), anteiso-C17: 0(17.2%) and iso-C16: 0(11.4%). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).
UR - http://www.scopus.com/inward/record.url?scp=84925296589&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.000053
DO - 10.1099/ijs.0.000053
M3 - Article
C2 - 25563924
AN - SCOPUS:84925296589
SN - 1466-5026
VL - 65
SP - 996
EP - 1002
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 3
ER -