Serotonergic projections from the nucleus raphe dorsalis to the amygdala in the rat

Q. P. Ma; G. F. Yin; M. K. Ai; J. S. Han

doi:10.1016/0304-3940(91)90499-J

Serotonergic projections from the nucleus raphe dorsalis to the amygdala in the rat

Q. P. Ma, G. F. Yin, M. K. Ai, J. S. Han

Research output: Journal Publication › Article › peer-review

109 Citations (Scopus)

Abstract

Previous studies have shown a possible connection between the nucleus raphe dorsalis (NRD) and the amygdala in mediating opioid analgesia. In the present study, horseradish peroxidase (HRP) retrograde tracing was used in combination with serotonin (5-HT) immunocytochemical staining in an attempt to search for serotonergic projections from the NRD to the amygdala. In rats which received an injection of HRP into the amygdala, HRP retrogradely labelled 5-HT-immunoreactive cells were observed in the NRD. About 10% of the 5-HT-immunoreactive neurons in the NRD give rise to axons to the amygdala. These cells are predominantly situated in the ipsilateral wing and ventromedial part of the NRD. These data indicate the existence of serotonergic projections from the NRD to the amygdala, providing a morphological substrate for the putative antinociceptive pathway from the NRD to the amygdala.

Original language	English
Pages (from-to)	21-24
Number of pages	4
Journal	Neuroscience Letters
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90499-J
Publication status	Published - 16 Dec 1991
Externally published	Yes

Keywords

Nucleus raphe dorsalis
amygdala
horseradish peroxidase
immunocytochemistry
serotonin

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(91)90499-J

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title = "Serotonergic projections from the nucleus raphe dorsalis to the amygdala in the rat",

abstract = "Previous studies have shown a possible connection between the nucleus raphe dorsalis (NRD) and the amygdala in mediating opioid analgesia. In the present study, horseradish peroxidase (HRP) retrograde tracing was used in combination with serotonin (5-HT) immunocytochemical staining in an attempt to search for serotonergic projections from the NRD to the amygdala. In rats which received an injection of HRP into the amygdala, HRP retrogradely labelled 5-HT-immunoreactive cells were observed in the NRD. About 10% of the 5-HT-immunoreactive neurons in the NRD give rise to axons to the amygdala. These cells are predominantly situated in the ipsilateral wing and ventromedial part of the NRD. These data indicate the existence of serotonergic projections from the NRD to the amygdala, providing a morphological substrate for the putative antinociceptive pathway from the NRD to the amygdala.",

keywords = "Nucleus raphe dorsalis, amygdala, horseradish peroxidase, immunocytochemistry, serotonin",

author = "Ma, {Q. P.} and Yin, {G. F.} and Ai, {M. K.} and Han, {J. S.}",

note = "Funding Information: This work was supported by Grant DA03983 from the National Institute of Drug Abuse, U.S.A.",

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AU - Ma, Q. P.

AU - Yin, G. F.

AU - Ai, M. K.

AU - Han, J. S.

N1 - Funding Information: This work was supported by Grant DA03983 from the National Institute of Drug Abuse, U.S.A.

PY - 1991/12/16

Y1 - 1991/12/16

N2 - Previous studies have shown a possible connection between the nucleus raphe dorsalis (NRD) and the amygdala in mediating opioid analgesia. In the present study, horseradish peroxidase (HRP) retrograde tracing was used in combination with serotonin (5-HT) immunocytochemical staining in an attempt to search for serotonergic projections from the NRD to the amygdala. In rats which received an injection of HRP into the amygdala, HRP retrogradely labelled 5-HT-immunoreactive cells were observed in the NRD. About 10% of the 5-HT-immunoreactive neurons in the NRD give rise to axons to the amygdala. These cells are predominantly situated in the ipsilateral wing and ventromedial part of the NRD. These data indicate the existence of serotonergic projections from the NRD to the amygdala, providing a morphological substrate for the putative antinociceptive pathway from the NRD to the amygdala.

AB - Previous studies have shown a possible connection between the nucleus raphe dorsalis (NRD) and the amygdala in mediating opioid analgesia. In the present study, horseradish peroxidase (HRP) retrograde tracing was used in combination with serotonin (5-HT) immunocytochemical staining in an attempt to search for serotonergic projections from the NRD to the amygdala. In rats which received an injection of HRP into the amygdala, HRP retrogradely labelled 5-HT-immunoreactive cells were observed in the NRD. About 10% of the 5-HT-immunoreactive neurons in the NRD give rise to axons to the amygdala. These cells are predominantly situated in the ipsilateral wing and ventromedial part of the NRD. These data indicate the existence of serotonergic projections from the NRD to the amygdala, providing a morphological substrate for the putative antinociceptive pathway from the NRD to the amygdala.

KW - Nucleus raphe dorsalis

KW - amygdala

KW - horseradish peroxidase

KW - immunocytochemistry

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ER -

Serotonergic projections from the nucleus raphe dorsalis to the amygdala in the rat

Abstract

Keywords

ASJC Scopus subject areas

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