TY - JOUR
T1 - Preparation of diacylglycerols from Acer truncatum seed oil via enzymatic glycerolysis and evaluation of their neuroprotective effects
AU - Zhang, Shangkun
AU - Wu, Yuan
AU - Zhou, Yunkai
AU - Wang, Jia
AU - Li, Jian
AU - Li, Dongbing
N1 - Publisher Copyright:
© 2025 Elsevier Ltd
PY - 2025/7
Y1 - 2025/7
N2 - Acer truncatum seed oil (ATO), rich in nervonic acid, was enzymatically converted to diacylglycerols (DAG) via optimized glycerolysis (1:1 ATO:glycerol ratio, 2 % Novozym 435, 60 °C, 24 h), achieving a >50 % DAG yield. The process demonstrated scalability (50-fold) and enzyme reusability (6 cycles), yielding 50 % DAG content in scaled batches. Purification through molecular distillation and silica gel chromatography produced high-purity DAG (98.7 %) while preserving fatty acid composition. In H2O2-induced PC12 cells, ATO-derived DAG (40 μg mL−1) enhanced cell viability by 32 % (vs. H2O2 controls), reduced reactive oxygen species (ROS) by 49 %, lowered malondialdehyde (MDA) levels to 20.8 μM mg−1 protein, and restored the activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GPx). These results establish enzymatic glycerolysis as a scalable method for neuroprotective DAG production and position ATO-derived DAG as a promising therapeutic against oxidative neurodegeneration.
AB - Acer truncatum seed oil (ATO), rich in nervonic acid, was enzymatically converted to diacylglycerols (DAG) via optimized glycerolysis (1:1 ATO:glycerol ratio, 2 % Novozym 435, 60 °C, 24 h), achieving a >50 % DAG yield. The process demonstrated scalability (50-fold) and enzyme reusability (6 cycles), yielding 50 % DAG content in scaled batches. Purification through molecular distillation and silica gel chromatography produced high-purity DAG (98.7 %) while preserving fatty acid composition. In H2O2-induced PC12 cells, ATO-derived DAG (40 μg mL−1) enhanced cell viability by 32 % (vs. H2O2 controls), reduced reactive oxygen species (ROS) by 49 %, lowered malondialdehyde (MDA) levels to 20.8 μM mg−1 protein, and restored the activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GPx). These results establish enzymatic glycerolysis as a scalable method for neuroprotective DAG production and position ATO-derived DAG as a promising therapeutic against oxidative neurodegeneration.
KW - Acer truncatum seed oil
KW - Diacylglycerols
KW - Hydrogen peroxide injury
KW - Lipase catalysis
KW - Neuroprotection
UR - http://www.scopus.com/inward/record.url?scp=105006682519&partnerID=8YFLogxK
U2 - 10.1016/j.fbio.2025.106943
DO - 10.1016/j.fbio.2025.106943
M3 - Article
AN - SCOPUS:105006682519
SN - 2212-4292
VL - 69
JO - Food Bioscience
JF - Food Bioscience
M1 - 106943
ER -