Abstract
Acer truncatum seed oil (ATO), rich in nervonic acid, was enzymatically converted to diacylglycerols (DAG) via optimized glycerolysis (1:1 ATO:glycerol ratio, 2 % Novozym 435, 60 °C, 24 h), achieving a > 50 % DAG yield. The process demonstrated scalability (50-fold) and enzyme reusability (6 cycles), yielding 50 % DAG content in scaled batches. Purification through molecular distillation and silica gel chromatography produced high-purity DAG (98.7 %) while preserving fatty acid composition. In H2O2-induced PC12 cells, ATO-derived DAG (40 μg mL-1) enhanced cell viability by 32 % (vs. H2O2 controls), reduced reactive oxygen species (ROS) by 49 %, lowered malondialdehyde (MDA) levels to 20.8 μM mg-1 protein, and restored the activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GPx). These results establish enzymatic glycerolysis as a scalable method for neuroprotective DAG production and position ATO-derived DAG as a promising therapeutic against oxidative neurodegeneration.
Original language | English |
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Article number | 106943 |
Number of pages | 11 |
Journal | Food Bioscience |
Volume | 69 |
DOIs | |
Publication status | Published - Jul 2025 |
Keywords
- Acer truncatum seed oil
- diacylglycerols
- lipase catalysis
- hydrogen peroxide injury
- neuroprotection