Highly synergistic antibacterial activity of copper (II)-based nano metal–organic framework

Copper(II)-based metal–organic framework [Cu₂(C₆H₈O₇)(H₂O)₂]_n, bulk crystals (Micro-MOF) and nano-MOF particles (Nano-MOF) were synthesized via the hydrothermal method(cit = citric acid(organic linker). The Cu(II) centers of this micro-MOFs, according to a single crystal X-ray study, are surrounded by a 3D structural network and two different coordination environments. The size of micro-MOF was around 200 m, but the width of nano-MOF as-synthesized was close to 120 ± 20 nm. Zone of inhibition and minimal inhibitory concentration (MIC) tests were used to determine the antibacterial effectiveness of the nano-MOF and micro-MOF particle against Escherichia coli and Bacillus subtilis, respectively. While the MIC for micro-MOF is between 200 and 250 ppm and nano-MOF is between 150 and 200 ppm against E. coli and B. subtilis. respectively. The findings show that nano-MOF has more antibacterial power than micro-MOF and ligand by itself. Reactive oxygen species (ROS) and the delayed liberation of incorporating Cu²⁺ ions were the foundation for a synergistic antibacterial mechanism that was also put forward.