TY - JOUR
T1 - Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae
AU - Lee, Learn Han
AU - Cheah, Yoke Kqueen
AU - Sidik, Shiran Mohd
AU - Xie, Qing Yi
AU - Tang, Yi Li
AU - Lin, Hai Peng
AU - Ab Mutalib, Nurul Syakima
AU - Hong, Kui
PY - 2013
Y1 - 2013
N2 - Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9% 16S rRNA gene sequence similarity), Branchiibius (95.7%), Dermacoccus (94.4-95.3%), Calidifontibacter (94.6%), Luteipulveratus (94.3%), Yimella (94.2%) and Kytococcus (93.1%). Cells were irregular cocci and short rods. The peptidoglycan type was A4a with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17: 0 (41.97%), anteiso-C17: 1ω9c (32.16%) and iso-C16: 0 (7.68%). The DNA G+C content of strain 39T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T (=CGMCC 4.6864T =DSM 24617T).
AB - Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9% 16S rRNA gene sequence similarity), Branchiibius (95.7%), Dermacoccus (94.4-95.3%), Calidifontibacter (94.6%), Luteipulveratus (94.3%), Yimella (94.2%) and Kytococcus (93.1%). Cells were irregular cocci and short rods. The peptidoglycan type was A4a with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17: 0 (41.97%), anteiso-C17: 1ω9c (32.16%) and iso-C16: 0 (7.68%). The DNA G+C content of strain 39T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T (=CGMCC 4.6864T =DSM 24617T).
UR - http://www.scopus.com/inward/record.url?scp=84872477972&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.038232-0
DO - 10.1099/ijs.0.038232-0
M3 - Article
C2 - 22389286
AN - SCOPUS:84872477972
SN - 1466-5026
VL - 63
SP - 241
EP - 248
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 1
ER -