@inproceedings{6c6ad363e4714222b7534c2dd5b4cd8a,
title = "Applications of single cell profiles of PBMC:Improvements of cell type classification",
abstract = "Single-cell-derived-class (SCDC) profiles capture characteristic gene expression from single cells representing types and subtypes and their conditions. SCDC profiles show high reproducibility across similar single-cell types processed under the same conditions. We have demonstrated two applications of SCDC profiles-classification of single cells from PBMC into six main classes (B cells, cDC, pDC, monocytes, NK cells, and T cells) and into three super-classes (BC+pDC,MC+cDC, and TC+NK). The minimum number of individual cells required for building an effective reference SCDC profile has been assessed to be between 160 and 640 cells. The variability of SCDC gradually decreases as the number of cells used to derive the profile increases from 10-cells to 640-cells. The classification accuracy of PBMC extracted by PBMC separation by SCDC profiles was 85-100% and 95-100% for supertypes depending on the cell type or supertype. Classification accuracy for PBMC cell types is lower for samples that are processed by cell sorting, or other sample processing steps.",
keywords = "10x SCT, PBMC, classification, gene expression profiles, quantity control, super-class",
author = "Luning Yang and Yihan Zhang and Nenad Mitic and Keskin, {Derin B.} and Zhang, {Guang Lan} and Chitkushev, {Lou T.} and Richard Rankin and Vladimir Brusic",
note = "Publisher Copyright: {\textcopyright} 2021 IEEE.; 2021 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2021 ; Conference date: 09-12-2021 Through 12-12-2021",
year = "2021",
doi = "10.1109/BIBM52615.2021.9669678",
language = "English",
series = "Proceedings - 2021 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2021",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "3334--3340",
editor = "Yufei Huang and Lukasz Kurgan and Feng Luo and Hu, {Xiaohua Tony} and Yidong Chen and Edward Dougherty and Andrzej Kloczkowski and Yaohang Li",
booktitle = "Proceedings - 2021 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2021",
address = "United States",
}