Anionic powdered-nanoliposome loaded with algal oil: Effects of carrier agents on storage stability, in-vitro digestibility and cellular uptake

Yuanyuan Liu; Xiaoxiao Feng; Jiahe Zhao; Jiale Lin; Cai Shen; Oi-Ming Lai; Chin-Ping Tan; Ling-Zhi Cheong

doi:10.21203/rs.3.rs-1840648/v1

Anionic powdered-nanoliposome loaded with algal oil: Effects of carrier agents on storage stability, in-vitro digestibility and cellular uptake

Yuanyuan Liu, Xiaoxiao Feng, Jiahe Zhao, Jiale Lin, Cai Shen, Oi-Ming Lai, Chin-Ping Tan, Ling-Zhi Cheong

CBI Green Chemicals and Energy Centre

Research output: Other contribution

Abstract

Present study prepared anionic powdered-nanoliposomes loaded with algal oil using phosphatidylserine and different wall materials (maltodextrin and hydroxypropyl β-cyclodextrin). All the powdered liposomes were nanosized (238.97 ± 4.67–256.30 ± 0.85 nm) with negative zeta potential (-71.80 ± 7.84 – -51.46 ± 1.27 mv). We have investigated and compared the effects of different wall materials on the oxidative stability, in-vitro digestibility and cellular uptake of the spray-dried powders were investigated. Results showed MD was able to provide higher oxidative stability to anionic-powdered liposomes loaded with algal oil. In addition, powdered liposomes produced from MD demonstrated better in-vitro digestibility with increased in SLC27A4, FABP2 and CD36 gene expression indicating good cellular uptake of the powdered liposomes.

Original language	English
Publisher	Research Square Platform LLC
DOIs	https://doi.org/10.21203/rs.3.rs-1840648/v1 https://doi.org/10.21203/rs.3.rs-1840648/v1
Publication status	Published - 2022

Keywords

DHA
Digestion
Lipid uptake
Nanoliposome
Spray drying
Stability

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@misc{ed90cf15ce114c298d000a0706d67de1,

title = "Anionic powdered-nanoliposome loaded with algal oil: Effects of carrier agents on storage stability, in-vitro digestibility and cellular uptake",

abstract = "Present study prepared anionic powdered-nanoliposomes loaded with algal oil using phosphatidylserine and different wall materials (maltodextrin and hydroxypropyl β-cyclodextrin). All the powdered liposomes were nanosized (238.97 ± 4.67–256.30 ± 0.85 nm) with negative zeta potential (-71.80 ± 7.84 – -51.46 ± 1.27 mv). We have investigated and compared the effects of different wall materials on the oxidative stability, in-vitro digestibility and cellular uptake of the spray-dried powders were investigated. Results showed MD was able to provide higher oxidative stability to anionic-powdered liposomes loaded with algal oil. In addition, powdered liposomes produced from MD demonstrated better in-vitro digestibility with increased in SLC27A4, FABP2 and CD36 gene expression indicating good cellular uptake of the powdered liposomes.",

keywords = "DHA, Digestion, Lipid uptake, Nanoliposome, Spray drying, Stability",

author = "Yuanyuan Liu and Xiaoxiao Feng and Jiahe Zhao and Jiale Lin and Cai Shen and Oi-Ming Lai and Chin-Ping Tan and Ling-Zhi Cheong",

year = "2022",

doi = "10.21203/rs.3.rs-1840648/v1",

language = "English",

publisher = "Research Square Platform LLC",

type = "Other",

}

TY - GEN

T1 - Anionic powdered-nanoliposome loaded with algal oil: Effects of carrier agents on storage stability, in-vitro digestibility and cellular uptake

AU - Liu, Yuanyuan

AU - Feng, Xiaoxiao

AU - Zhao, Jiahe

AU - Lin, Jiale

AU - Shen, Cai

AU - Lai, Oi-Ming

AU - Tan, Chin-Ping

AU - Cheong, Ling-Zhi

PY - 2022

Y1 - 2022

N2 - Present study prepared anionic powdered-nanoliposomes loaded with algal oil using phosphatidylserine and different wall materials (maltodextrin and hydroxypropyl β-cyclodextrin). All the powdered liposomes were nanosized (238.97 ± 4.67–256.30 ± 0.85 nm) with negative zeta potential (-71.80 ± 7.84 – -51.46 ± 1.27 mv). We have investigated and compared the effects of different wall materials on the oxidative stability, in-vitro digestibility and cellular uptake of the spray-dried powders were investigated. Results showed MD was able to provide higher oxidative stability to anionic-powdered liposomes loaded with algal oil. In addition, powdered liposomes produced from MD demonstrated better in-vitro digestibility with increased in SLC27A4, FABP2 and CD36 gene expression indicating good cellular uptake of the powdered liposomes.

AB - Present study prepared anionic powdered-nanoliposomes loaded with algal oil using phosphatidylserine and different wall materials (maltodextrin and hydroxypropyl β-cyclodextrin). All the powdered liposomes were nanosized (238.97 ± 4.67–256.30 ± 0.85 nm) with negative zeta potential (-71.80 ± 7.84 – -51.46 ± 1.27 mv). We have investigated and compared the effects of different wall materials on the oxidative stability, in-vitro digestibility and cellular uptake of the spray-dried powders were investigated. Results showed MD was able to provide higher oxidative stability to anionic-powdered liposomes loaded with algal oil. In addition, powdered liposomes produced from MD demonstrated better in-vitro digestibility with increased in SLC27A4, FABP2 and CD36 gene expression indicating good cellular uptake of the powdered liposomes.

KW - DHA

KW - Digestion

KW - Lipid uptake

KW - Nanoliposome

KW - Spray drying

KW - Stability

U2 - 10.21203/rs.3.rs-1840648/v1

DO - 10.21203/rs.3.rs-1840648/v1

M3 - Other contribution

PB - Research Square Platform LLC

ER -

Anionic powdered-nanoliposome loaded with algal oil: Effects of carrier agents on storage stability, in-vitro digestibility and cellular uptake

Abstract

Keywords

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