An investigation on plant cell walls during biomass pyrolysis: A histochemical perspective on engineering applications

Yoong Xin Pang; Nusrat Sharmin; Tao Wu; Cheng Heng Pang

doi:10.1016/j.apenergy.2023.121055

An investigation on plant cell walls during biomass pyrolysis: A histochemical perspective on engineering applications

Yoong Xin Pang, Nusrat Sharmin, Tao Wu, Cheng Heng Pang

Research output: Journal Publication › Article › peer-review

1 Citation (Scopus)

Abstract

The large variation in biomass species, and hence lignocellulosic composition, often results in varying properties and production of pyrolytic products, i.e. bio-char, bio-oil and bio-gas. Hence, understanding of the distribution of individual lignocellulosic components in plant cell walls and its influence on cell structural evolution during pyrolysis is essential to predict biomass behaviour for different engineering applications. Bamboo shoot sections were subjected to histochemical safranin O/fast green solution staining to reveal the distribution of lignocellulosic components in the sample. Stained fresh samples were compared against pyrolysed samples under light and oil immersion microscopy to investigate the structural evolution of primary and secondary cell walls during pyrolysis. Charred samples were subsequently characterised via BET, XRD and Raman spectroscopy to further understand the influence of pyrolytic temperatures on cell transformations. This study provides insights into understanding the devolatilisation and graphitisation characteristics of individual plant cell wall and its lignocellulosic components, thus facilitating biomass selection for targeted applications in energy derivation and/or material engineering without the need of repeating chemical-intensive histochemical and pyrolysis procedures.

Original language	English
Article number	121055
Journal	Applied Energy
Volume	343
DOIs	https://doi.org/10.1016/j.apenergy.2023.121055
Publication status	Published - 1 Aug 2023

Keywords

Image analysis
Lignocellulose
Microscopy
Pyrolytic products
Safranin O/fast green

ASJC Scopus subject areas

Building and Construction
Renewable Energy, Sustainability and the Environment
Mechanical Engineering
General Energy
Management, Monitoring, Policy and Law

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.apenergy.2023.121055

Cite this

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title = "An investigation on plant cell walls during biomass pyrolysis: A histochemical perspective on engineering applications",

abstract = "The large variation in biomass species, and hence lignocellulosic composition, often results in varying properties and production of pyrolytic products, i.e. bio-char, bio-oil and bio-gas. Hence, understanding of the distribution of individual lignocellulosic components in plant cell walls and its influence on cell structural evolution during pyrolysis is essential to predict biomass behaviour for different engineering applications. Bamboo shoot sections were subjected to histochemical safranin O/fast green solution staining to reveal the distribution of lignocellulosic components in the sample. Stained fresh samples were compared against pyrolysed samples under light and oil immersion microscopy to investigate the structural evolution of primary and secondary cell walls during pyrolysis. Charred samples were subsequently characterised via BET, XRD and Raman spectroscopy to further understand the influence of pyrolytic temperatures on cell transformations. This study provides insights into understanding the devolatilisation and graphitisation characteristics of individual plant cell wall and its lignocellulosic components, thus facilitating biomass selection for targeted applications in energy derivation and/or material engineering without the need of repeating chemical-intensive histochemical and pyrolysis procedures.",

keywords = "Image analysis, Lignocellulose, Microscopy, Pyrolytic products, Safranin O/fast green",

author = "Pang, {Yoong Xin} and Nusrat Sharmin and Tao Wu and Pang, {Cheng Heng}",

note = "Funding Information: The authors gratefully express gratitude to all parties who have contributed towards the success of this project, both financially and technically, especially the S&T Innovation 2025 Major Special Programme [Grant number 2018B10022] and the Ningbo Commonweal Programme [Grant number 2022S122] funded by the Ningbo Science and Technology Bureau, China, as well as the UNNC FoSE Faculty Inspiration Grant, China. The authors would like to acknowledge the support from the Ningbo Municipal Key Laboratory on Clean Energy Conversion Technologies [2014A22010] as well as the Zhejiang Provincial Key Laboratory for Carbonaceous Wastes Processing and Process Intensification Research funded by the Zhejiang Provincial Department of Science and Technology [2020E10018]. Publisher Copyright: {\textcopyright} 2023 The Authors",

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doi = "10.1016/j.apenergy.2023.121055",

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T1 - An investigation on plant cell walls during biomass pyrolysis

T2 - A histochemical perspective on engineering applications

AU - Pang, Yoong Xin

AU - Sharmin, Nusrat

AU - Wu, Tao

AU - Pang, Cheng Heng

N1 - Funding Information: The authors gratefully express gratitude to all parties who have contributed towards the success of this project, both financially and technically, especially the S&T Innovation 2025 Major Special Programme [Grant number 2018B10022] and the Ningbo Commonweal Programme [Grant number 2022S122] funded by the Ningbo Science and Technology Bureau, China, as well as the UNNC FoSE Faculty Inspiration Grant, China. The authors would like to acknowledge the support from the Ningbo Municipal Key Laboratory on Clean Energy Conversion Technologies [2014A22010] as well as the Zhejiang Provincial Key Laboratory for Carbonaceous Wastes Processing and Process Intensification Research funded by the Zhejiang Provincial Department of Science and Technology [2020E10018]. Publisher Copyright: © 2023 The Authors

PY - 2023/8/1

Y1 - 2023/8/1

N2 - The large variation in biomass species, and hence lignocellulosic composition, often results in varying properties and production of pyrolytic products, i.e. bio-char, bio-oil and bio-gas. Hence, understanding of the distribution of individual lignocellulosic components in plant cell walls and its influence on cell structural evolution during pyrolysis is essential to predict biomass behaviour for different engineering applications. Bamboo shoot sections were subjected to histochemical safranin O/fast green solution staining to reveal the distribution of lignocellulosic components in the sample. Stained fresh samples were compared against pyrolysed samples under light and oil immersion microscopy to investigate the structural evolution of primary and secondary cell walls during pyrolysis. Charred samples were subsequently characterised via BET, XRD and Raman spectroscopy to further understand the influence of pyrolytic temperatures on cell transformations. This study provides insights into understanding the devolatilisation and graphitisation characteristics of individual plant cell wall and its lignocellulosic components, thus facilitating biomass selection for targeted applications in energy derivation and/or material engineering without the need of repeating chemical-intensive histochemical and pyrolysis procedures.

AB - The large variation in biomass species, and hence lignocellulosic composition, often results in varying properties and production of pyrolytic products, i.e. bio-char, bio-oil and bio-gas. Hence, understanding of the distribution of individual lignocellulosic components in plant cell walls and its influence on cell structural evolution during pyrolysis is essential to predict biomass behaviour for different engineering applications. Bamboo shoot sections were subjected to histochemical safranin O/fast green solution staining to reveal the distribution of lignocellulosic components in the sample. Stained fresh samples were compared against pyrolysed samples under light and oil immersion microscopy to investigate the structural evolution of primary and secondary cell walls during pyrolysis. Charred samples were subsequently characterised via BET, XRD and Raman spectroscopy to further understand the influence of pyrolytic temperatures on cell transformations. This study provides insights into understanding the devolatilisation and graphitisation characteristics of individual plant cell wall and its lignocellulosic components, thus facilitating biomass selection for targeted applications in energy derivation and/or material engineering without the need of repeating chemical-intensive histochemical and pyrolysis procedures.

KW - Image analysis

KW - Lignocellulose

KW - Microscopy

KW - Pyrolytic products

KW - Safranin O/fast green

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DO - 10.1016/j.apenergy.2023.121055

M3 - Article

AN - SCOPUS:85159125779

SN - 0306-2619

VL - 343

JO - Applied Energy

JF - Applied Energy

M1 - 121055

ER -

An investigation on plant cell walls during biomass pyrolysis: A histochemical perspective on engineering applications

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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