Thapsigargin-coated intraocular lenses inhibit human lens cell growth

G. Duncan, I. M. Wormstone, C. S.C. Liu, J. M. Marcantonio, P. D. Davies

Research output: Journal PublicationArticlepeer-review

115 Citations (Scopus)


Cataract is responsible for rendering several million people blind throughout the world1 and is also by far the most common cause of low visual acuity. Although cataract surgery is common, routine and effective, posterior capsule opacification (PCO) occurs in 30-50% of patients following modern cataract surgery. This condition arises from stimulated cell growth within the capsular bag after surgery2. The resulting decline in visual acuity requires expensive laser treatment, and PCO therefore prevents modern cataract surgery from being carried out routinely in underdeveloped countries. The present study, using a human lens capsular bag culture system3, has confirmed that cells from a wide age range of donors proliferate in the absence of added serum protein4 and explains why PCO is such a common problem even in aged patients. This study also provides one possible solution for PCO by using polymethylmethacrylate (PMMA) implanted intraocular lenses as a drug delivery system. PMMA lenses coated with thapsigargin, a hydrophobic inhibitor of endoplasmic reticulum (ER) (Ca2)ATPase5, greatly reduced cell growth in the capsular bag at relatively low coating concentrations (200 nM) but, more significantly, induced total cell death of the residual anterior epithelial cells at higher concentrations (>2 μM).

Original languageEnglish
Pages (from-to)1026-1028
Number of pages3
JournalNature Medicine
Issue number9
Publication statusPublished - Sept 1997
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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