Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach

Ali Mohsin, Jingyun Sun, Imran Mahmood Khan, Haifeng Hang, Muhammad Tariq, Xiwei Tian, Waqas Ahmed, Sobia Niazi, Yingping Zhuang, Ju Chu, Muhammad Zubair Mohsin, Salim-ur-Rehman, Meijin Guo

Research output: Journal PublicationArticlepeer-review

34 Citations (Scopus)

Abstract

This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60–120 °C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95–98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate (μm = 0.233/h), highest curdlan production (Pm = 23.24 g/L) and growth associated rate constant (α = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.

Original languageEnglish
Pages (from-to)626-635
Number of pages10
JournalCarbohydrate Polymers
Volume205
DOIs
Publication statusPublished - 1 Feb 2019
Externally publishedYes

Keywords

  • Batch culture fermentation
  • Curdlan
  • Kinetics
  • Orange peels
  • Sustainable biosynthesis

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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