Screening and application of a broad-spectrum aptamer for acyclic guanosine analogues

Le Ren, Shuo Qi, Imran Mahmood Khan, Shijia Wu, Nuo Duan, Zhouping Wang

Research output: Journal PublicationArticlepeer-review

7 Citations (Scopus)

Abstract

Acyclic guanosine analogues, a class of widely used antiviral drugs, can cause chronic toxicity and virus resistance. Therefore, it is essential to establish rapid and accurate methods to detect acyclic guanosine analogues. In this study, five acyclic guanosine analogues (acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir) were used as positive targets to obtain broad-spectrum aptamers through Capture-SELEX technology. Real-time quantitative PCR (Q-PCR) was used to monitor the aptamer SELEX process. After the sixteen rounds of selection against mixed targets, sequences were obtained by high-throughput sequencing (HTS). Furthermore, a broad-spectrum aptamer, named CIV6, was found as the higher performance aptamer that was suitable for five acyclic guanosine analogues by graphene oxide (GO) polarization and fluorescence assay. Finally, the aptamer CIV6 was used to construct GO fluorescence assay to detect five acyclic guanosine analogues. The limits of detection (LOD) of acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir were 0.48 ng·mL−1, 0.53 ng·mL−1, 0.50 ng·mL−1, 0.56 ng·mL−1, and 0.38 ng·mL−1, respectively. Graphical abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)4855-4863
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume413
Issue number19
DOIs
Publication statusPublished - Aug 2021
Externally publishedYes

Keywords

  • Acyclic guanosine analogues
  • Aptamer
  • Capture-SELEX
  • Fluorescence
  • Polarization

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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