Rebuilding the lid region from conformational and dynamic features to engineering applications of lipase in foods: Current status and future prospects

Gang Chen, Imran Mahmood Khan, Wensen He, Yongxin Li, Peng Jin, Osvaldo H. Campanella, Haihua Zhang, Yanrong Huo, Yang Chen, Huqing Yang, Ming Miao

Research output: Journal PublicationArticlepeer-review

22 Citations (Scopus)

Abstract

The applications of lipases in esterification, amidation, and transesterification have broadened their potential in the production of fine compounds with high cumulative values. Mostly, the catalytic triad of lipases is covered by either one or two mobile peptides called the “lid” that control the substrate channel to the catalytic center. The lid holds unique conformational allostery via interfacial activation to regulate the dynamics and catalytic functions of lipases, thereby highlighting its importance in redesigning these enzymes for industrial applications. The structural characteristic of lipase, the dynamics of lids, and the roles of lid in lipase catalysis were summarized, providing opportunities for rebuilding lid region by biotechniques (e.g., metagenomic technology and protein engineering) and enzyme immobilization. The review focused on the advantages and disadvantages of strategies rebuilding the lid region. The main shortcomings of biotechnologies on lid rebuilding were discussed such as negative effects on lipase (e.g., a decrease of activity). Additionally, the main shortcomings (e.g., enzyme desorption at high temperatre) in immobilization on hydrophobic supports via interfacial action were presented. Solutions to the mentioned problems were proposed by combinations of computational design with biotechnologies, and improvements of lipase immobilization (e.g., immobilization protocols and support design). Finally, the review provides future perspectives about designing hyperfunctional lipases as biocatalysts in the food industry based on lid conformation and dynamics.

Original languageEnglish
Pages (from-to)2688-2714
Number of pages27
JournalComprehensive Reviews in Food Science and Food Safety
Volume21
Issue number3
DOIs
Publication statusPublished - May 2022
Externally publishedYes

Keywords

  • allosteric effects
  • enzymatic catalysis
  • food industry
  • lipase lid
  • rebuilding approaches

ASJC Scopus subject areas

  • Food Science

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