Pterygial derived fibroblasts express functionally active histamine and epidermal growth factor receptors

Raj Maini, David J. Collison, Jill M. Maidment, Peter D. Davies, I. Michael Wormstone

Research output: Journal PublicationArticlepeer-review

32 Citations (Scopus)

Abstract

Pterygia are characterised by a fleshy outgrowth of altered conjuctival tissue over the cornea and are most common in tropical regions. Pterygial fibroblasts are characteristically distinct from normal conjunctival fibroblasts, and therefore the aim of this study was to determine the presence and functional significance of histamine and epidermal growth factor (EGF) receptors in these cells. Pterygial specimens were cultured in vitro and cellular outgrowths were phenotypically characterised as fibroblasts using vimentin and cytokeratin staining. Intracellular calcium mobilization was used to characterise the functional activity of histamine receptors on these cells. Maximal response was obtained with 100 μM histamine. However, lower concentrations of histamine also caused mobilization of calcium that were totally abolished by pre-incubation with H1 but not H2 or H3 receptor antagonists. EGF receptor was diffusely expressed over the cell surfaces. EGF stimulated receptor internalization, ERK protein phosphorylation and intracellular calcium mobilization. Therefore, fibroblasts derived from human pterygia express functionally active histamine and epidermal growth factor receptors. Controlled modification of either the receptors or the appropriate ligands could have beneficial effects in pterygia treatment.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalExperimental Eye Research
Volume74
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Calcium
  • EGF
  • ERK
  • Fibroblasts
  • Histamine
  • Human
  • Internalization
  • Pterygia
  • Receptors

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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