Protective effects of aerosolized pulmonary surfactant powder in a model of ventilator-induced lung injury

Derek Daniher, Lynda McCaig, Yuqing Ye, Ruud Veldhuizen, James Lewis, Yingliang Ma, Jesse Zhu

Research output: Journal PublicationArticlepeer-review

3 Citations (Scopus)

Abstract

Mechanical ventilation may contribute to the impairment of the pulmonary surfactant system, which is one of the mechanisms leading to the progression of acute lung injury. To investigate the potential protective effects of pulmonary surfactant in a rat model of ventilator-induced lung injury, the surfactant powder was aerosolized using an in-house made device designed to deliver the aerosolized powder to the inspiratory line of a rodent ventilator circuit. Rats were randomized to (i) administration of aerosolized recombinant surfactant protein C based pulmonary surfactant, (ii) intratracheally instillation of the same surfactant re-constituted in saline, and (iii) no treatment. Animals were monitored during 2 h of high-tidal volume mechanical ventilation, after which rats were sacrificed, and further analysis of lung mechanics and surfactant function were completed. Blood gas measurements during ventilation showed extended maintenance of oxygen levels above 400 mmHg in aerosol treated animals over non-treated and instilled groups, while total protein analysis showed reduced levels in the aerosol compared to non-treated groups. Dynamic captive bubble surface tension measurements showed the activity of surfactant recovered from aerosol treated animals is maintained below 1 mN/m. The prophylactic treatment of aerosolized surfactant powder reduced the severity of lung injury in this model.

Original languageEnglish
Article number119359
JournalInternational Journal of Pharmaceutics
Volume583
DOIs
Publication statusPublished - 15 Jun 2020
Externally publishedYes

Keywords

  • Aerosol
  • Dry powder inhaler
  • Mechanical ventilation
  • Pulmonary surfactant

ASJC Scopus subject areas

  • Pharmaceutical Science

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