Novel metal enhanced dual-mode fluorometric and SERS aptasensor incorporating a heterostructure nanoassembly for ultrasensitive T-2 toxin detection

Imran Mahmood Khan, Sobia Niazi, Imran Pasha, Muhammad Kashif Iqbal Khan, Lin Yue, Hua Ye, Ali Mohsin, Muhammad Shoaib, Yin Zhang, Zhouping Wang

Research output: Journal PublicationArticlepeer-review

1 Citation (Scopus)

Abstract

Fluorescent gold (Au) nanostructures have emerged as burgeoning materials to fabricate nanomaterial assemblies which play a vital role in improving the detection sensitivity and specificity for various biomolecules. In this work, a fluorescence labelled (Rhodamine-B-Isothiocyanate) silica shell with Au metal core (AuNPs@PVP@RITC@SiO2) and a graphene-Au nanostar nanocomposite (rGO-AuNS) are presented as a metal enhanced fluorescence (MEF) material and Raman signal enhancer, respectively. Their composite (AuNPs@PVP@RITC@SiO2NPs/rGO-AuNS) was employed as a dual-mode fluorescence (FL) and surface-enhanced Raman scattering (SERS) nanoprobe for selective and sensitive detection of T-2 toxin. To comprehend the dual-modality, a core-shell nanostructure, AuNPs@PVP@RITC@SiO2, was functionalized with an aptamer (donor) and adsorbed on the surface of rGO-AuNS through electrostatic forces and π-π stacking which act as a FL quencher and SERS signal enhancer. When exposed to T-2 toxin, the apt-AuNPs@PVP@RITC@SiO2NPs move away from the surface of rGO-AuNS, resulting in the restoration of FL and reduction of the SERS signal. There was distinct linearity between the T-2 toxin concentration and the dual FL and SERS signals with lower limits of detection (LOD) of 85 pM and 12 pM, as compared to the previous methods, respectively. The developed FL and SERS aptasensor presented excellent recovery ratio and RSD in wheat and maize, respectively, as compared with the standard ELISA method. The complementary performances of the developed stratagem revealed a high correlation between the FL and SERS sensing modes with exquisite detection properties.

Original languageEnglish
Pages (from-to)441-451
Number of pages11
JournalJournal of Materials Chemistry B
Volume11
Issue number2
DOIs
Publication statusPublished - 16 Nov 2022
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • Biomedical Engineering
  • General Materials Science

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