Monitoring the direct and indirect damage of DNA bases and polynucleotides by using time-resolved infrared spectroscopy

Marina K. Kuimova, Alexander J. Cowan, Pavel Matousek, Anthony W. Parker, Xue Zhong Sun, Michael Towrie, Michael W. George

Research output: Journal PublicationArticlepeer-review

59 Citations (Scopus)

Abstract

The nucleotide 5′-dGMP and polynucleotide poly(dGdC)-poly(dGdC) have been irradiated by using a 200-fs, 200-nm laser pulses and spectrally characterized by using time-resolved infrared spectroscopy. Under the experimental conditions, 200-nm excitation generates both electronic excited states and radical cations through photoionization; the former decay rapidly to vibrationally hot ground state. By using infrared signatures we have been able to follow these processes, and at time scales of >1 ns we observe an infrared marker band at 1,702 cm-1 within both 5′-dGMP and the polynucleotide assigned to a photoionized product of guanine. This transient has also been reproduced through indirect chemistry through the reaction with photogenerated carbonate radical with 5′-dGMP. The ability to use time-resolved infrared spectroscopy in this way paves the way for developing solution-phase studies to investigate both direct and indirect radiation chemistry of DNA.

Original languageEnglish
Pages (from-to)2150-2153
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number7
DOIs
Publication statusPublished - 14 Feb 2006
Externally publishedYes

Keywords

  • DNA damage
  • Electron transfer
  • Guanine radical cation

ASJC Scopus subject areas

  • General

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