Modified QuEChERS extraction method followed by simultaneous quantitation of nine multi-class pesticides in human blood and urine by using GC-MS

Shahid Iqbal, Muhammad Muntazir Iqbal, Mohsin Javed, Ali Bahadur, Sara Yasien, Najam-ud-din, Amir Hurr, Naveed Ahmad, Muhammad Raheel, Guocong Liu

Research output: Journal PublicationArticlepeer-review

40 Citations (Scopus)

Abstract

Organophosphate, carbamate and pyrethroid pesticides are the most common insecticides used worldwide. They may cause chronic poisoning in farmers and acute poisoning in homicidal or suicidal cases. The determination of trace levels of these pesticides in human blood and urine is very challenging. This study focuses on a simultaneous quantitation method that was developed and validated for multi-class nine pesticides belonging to organophosphate, carbamate and pyrethroid classes in human blood and urine. Target pesticides were extracted from blood and urine using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction method. Capillary column DB-35 ms (15 m × 0.25 mm, 0.25 µm) was used for chromatography with a 0.079 ml/min flow rate of carrier gas at constant pressure mode. Quantitation of sulfotep, phorate, carbofuran, chlorpyriphos, profenophos, triazophos, pyriproxyfen, lambda-cyhalothrin and permethrin was performed by mass spectrometer equipped with electron impact ionization source using selected ion monitoring (SIM) mode. The lower and upper limits of quantitation for all nine pesticides were 0.01 mg/L and 2.0 mg/dL respectively. The proposed method was proved to be simple, fast, sensitive, and robust. It has been applied to the analysis of 9 pesticides samples.

Original languageEnglish
Article number122227
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1152
DOIs
Publication statusPublished - 1 Sept 2020
Externally publishedYes

Keywords

  • GC-MS
  • Human blood and urine
  • Multi-class pesticides
  • Quantitation
  • QuEChERS extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Modified QuEChERS extraction method followed by simultaneous quantitation of nine multi-class pesticides in human blood and urine by using GC-MS'. Together they form a unique fingerprint.

Cite this