Improved method to express enzyme activity of Cx-enzyme

Jian Liu, Dong Bing Li, Ji Chu Yang

Research output: Contribution to specialist publicationArticle


Cellulase system contains a series of complex components, in which Cx-enzyme is a main member. Traditional assaying method of enzyme activity leads to lots of system mistakes attributable to low and alternative substrate concentration. On account of that, a reasonable new way to show enzyme activity by determining vmax is brought out. The research of this method focuses on the analysis of enzyme activity of Cx-enzyme expressed by Trichoderma koningii AS3.4262 in different concentrations of substrate. The data have been calculated under multiple regression to get initial reaction rates of different substrate concentrations. The values of constant (Km) and maximum reaction rate (vmax) are obtained by using Lineweaver-Burk chart. The benefit of this improved method is to express enzyme activity regardless of varied substrate concentrations.

Original languageEnglish
Number of pages3
Specialist publicationLinchan Huaxue Yu Gongye/Chemistry and Industry of Forest Products
Publication statusPublished - Dec 2005
Externally publishedYes


  • C-enzyme
  • CMC-Na
  • Enzyme activity
  • Trichoderma koningii

ASJC Scopus subject areas

  • Forestry
  • Fluid Flow and Transfer Processes


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