Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides

Yongfa Cheng, Baokai Ma, Chin Ping Tan, Oi Ming Lai, Worawan Panpipat, Ling Zhi Cheong, Cai Shen

Research output: Journal PublicationArticlepeer-review

24 Citations (Scopus)

Abstract

Free biocatalyst such as lipase cannot be recovered and reused; and is extremely unstable in harsh reaction condition. Present study aimed to synthesize hierarchical ordered macro-microporous ZIF-8 structures (MAC-ZIF-8) for encapsulation of nano-Burkholderia cepacia lipase (nano BCL). The synthesized MAC-ZIF-8 has good crystallinity, thermal stability and high surface area which made it suitable for immobilization of nano-BCL. The MAC-ZIF-8 immobilized nano-BCL (nano-BCL@MAC-ZIF-8) demonstrated high enzyme activity (4.3778 U) and can be recovered and reused for consecutive hydrolysis reaction. It is found that the nano-BCL is well encapsulated with the macropores of the MAC-ZIF-8 structures and they are able to preserve their native configuration with high affinity for substrates (lower Km of 1.068 mM as compared to that of nano-BCL@ZIF-8). The nano-BCL@MAC-ZIF-8 can also be used to fabricate electrochemical biosensors for detection of nitrogenous diphenyl ether pesticides (nitrofen) in real food systems (apricots). The fabricated biosensors demonstrated wide linear range (0−114µM), low limit of detection (0.46µM) and good recovery rate. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, low cost, and better linear range.

Original languageEnglish
Article number128477
JournalSensors and Actuators, B: Chemical
Volume321
DOIs
Publication statusPublished - 15 Oct 2020
Externally publishedYes

Keywords

  • Burkholderia cepacia lipase
  • Electrochemical detection
  • Immobilization
  • Lipase nanogel
  • Nitrogenous diphenyl ether pesticides
  • ZIF-8

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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