Abstract
Direct observation of J-couplings remains a challenge in high-resolution solid-state NMR. In some cases, it is possible to use Lee-Goldburg (LG) homonuclear decoupling during rare spin observation in MAS NMR correlation spectroscopy of lipid membranes to obtain J-resolved spectra in the direct dimension. In one simple implementation, a wide line separation-type 13C-1H HETCOR can provide high-resolution 1H/13C spectra, which are J-resolved in both dimensions. Coupling constants, 1JHC, obtained from 1H doublets, can be compared with scaled 1JCH-values obtained from the 13C multiplets to assess the LG efficiency and scaling factor. The use of homonuclear decoupling during proton evolution, LG-HETCOR-LG, can provide J-values, at least in the rare spin dimension, and allows measurements in less mobile membrane environments. The LG-decoupled spectroscopic approach is demonstrated on pure dioleoylphosphatidylcholine (DOPC) membranes and used to investigate lipid mixtures of DOPC/cholesterol and DOPC/cholesterol/sphingomyelin.
Original language | English |
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Pages (from-to) | 925-934 |
Number of pages | 10 |
Journal | Magnetic Resonance in Chemistry |
Volume | 48 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2010 |
Externally published | Yes |
Keywords
- HETCOR
- high-resolution MAS
- J-coupling
- Lee-Goldburg decoupling
- lipid membrane
- solid-state NMR
ASJC Scopus subject areas
- Chemistry (all)
- Materials Science (all)