Heteronuclear chemical shift correlation and J-resolved MAS NMR spectroscopy of lipid membranes

Vadim Zorin, Filip Ciesielski, David C. Griffin, Michael Rittig, Boyan B. Bonev

Research output: Journal PublicationArticlepeer-review

12 Citations (Scopus)


Direct observation of J-couplings remains a challenge in high-resolution solid-state NMR. In some cases, it is possible to use Lee-Goldburg (LG) homonuclear decoupling during rare spin observation in MAS NMR correlation spectroscopy of lipid membranes to obtain J-resolved spectra in the direct dimension. In one simple implementation, a wide line separation-type 13C-1H HETCOR can provide high-resolution 1H/13C spectra, which are J-resolved in both dimensions. Coupling constants, 1JHC, obtained from 1H doublets, can be compared with scaled 1JCH-values obtained from the 13C multiplets to assess the LG efficiency and scaling factor. The use of homonuclear decoupling during proton evolution, LG-HETCOR-LG, can provide J-values, at least in the rare spin dimension, and allows measurements in less mobile membrane environments. The LG-decoupled spectroscopic approach is demonstrated on pure dioleoylphosphatidylcholine (DOPC) membranes and used to investigate lipid mixtures of DOPC/cholesterol and DOPC/cholesterol/sphingomyelin.

Original languageEnglish
Pages (from-to)925-934
Number of pages10
JournalMagnetic Resonance in Chemistry
Issue number12
Publication statusPublished - Dec 2010
Externally publishedYes


  • J-coupling
  • Lee-Goldburg decoupling
  • high-resolution MAS
  • lipid membrane
  • solid-state NMR

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science


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