Fabrication of PAA coated green-emitting AuNCs for construction of label-free FRET assembly for specific recognition of T-2 toxin

Imran Mahmood Khan, Sobia Niazi, Ye Yu, Imran Pasha, Lin Yue, Ali Mohsin, Muhammad Shoaib, Muhammad Waheed Iqbal, Adnan Khaliq, Zhouping Wang

Research output: Journal PublicationArticlepeer-review

28 Citations (Scopus)

Abstract

In view of numerous encouraging requisitions, toxins quantification is an imperative research field of food safety. Herein, we synthesized a green-emitting L-arginine@6-aza-2-thiothymine-protected gold nanocluster (Arg@ATT-AuNCs) through host-guest assemblages. The Arg@ATT-AuNCs were capped with polyacrylic acid (PAA) to construct the PAA@Arg@ATT-AuNCs nanoparticles (NPs). The fluorescent and visual detection of T-2 (trichothecenes A) is based on PAA@Arg@ATT-AuNCs NPs and PDDA-aggregated-AuNPs assembly. In the absence of a target, electrostatic interaction of the aptamer-PDDA complex prevents the aggregation of AuNPs. The scattered AuNPs (absorbance at 520 nm) can quench the fluorescence intensity (F.I) of PAA@Arg@ATT-AuNCs NPs (emission at 530 nm) following FRET (Fluorescence resonance energy transfer) mechanism. The target addition leads in toxin-aptamer assemblies and succeeding PDDA is free to aggregate AuNPs (absorbance at 665 nm), resulted in recovered F.I. The developed bioassay sensitively used for quantification of T-2 with LOD of 0.57 pg mL−1 (0.001−100 ng mL−1). Spiking analysis reveals the robustness and efficiency of bioassay in maize and highly correlated with the standard ELISA method. We envision that the present study will substantively contribute to synthesize new core-shell NCs and may be considered as a new method for onsite detection.

Original languageEnglish
Article number128470
JournalSensors and Actuators, B: Chemical
Volume321
DOIs
Publication statusPublished - 15 Oct 2020
Externally publishedYes

Keywords

  • Aggregation
  • Aptmer-T-2 toxin complex
  • Dispersion
  • FRET
  • Gold nanoclusters
  • Gold nanoparticles

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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