Expression of capsaicin receptor (VR1) by myelinated primary afferent neurons in rats

Qing Ping Ma

doi:10.1016/S0304-3940(01)02537-X

Expression of capsaicin receptor (VR1) by myelinated primary afferent neurons in rats

Qing Ping Ma

Research output: Journal Publication › Article › peer-review

103 Citations (Scopus)

Abstract

The expression of capsaicin (VR1) receptor by A-fiber primary afferent neurons has been investigated by double immunohistochemical staining with VR1 and 200 kD neurofilament (NF200; an A-fiber marker) antibodies, and by VR1 immunohistochemical staining in combination with cholera toxin B subunit (CTB; also an A-fiber marker) retrograde tracing. Approximately 30% of the VR1-positive dorsal root ganglion (DRG) neurons were NF200-positive. Intra-sciatic nerve injection of CTB labeled over 30% of the VR1-positive neurons in the L5 DRG. Size frequency distribution analysis revealed that these VR1 and NF200, or VR1 and CTB, double-labeled neurons were predominantly small and medium sized. These results suggest that capsaicin receptors are likely to be expressed by Aδ-fiber neurons as well as C-fiber neurons.

Original language	English
Pages (from-to)	87-90
Number of pages	4
Journal	Neuroscience Letters
Volume	319
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(01)02537-X
Publication status	Published - 15 Feb 2002
Externally published	Yes

Keywords

Aδ-fibers
C-fibers
Capsaicin
Cholera toxin
Dorsal root ganglia
Neurofilament
VR1 receptor

ASJC Scopus subject areas

General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3940(01)02537-X

Cite this

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title = "Expression of capsaicin receptor (VR1) by myelinated primary afferent neurons in rats",

abstract = "The expression of capsaicin (VR1) receptor by A-fiber primary afferent neurons has been investigated by double immunohistochemical staining with VR1 and 200 kD neurofilament (NF200; an A-fiber marker) antibodies, and by VR1 immunohistochemical staining in combination with cholera toxin B subunit (CTB; also an A-fiber marker) retrograde tracing. Approximately 30% of the VR1-positive dorsal root ganglion (DRG) neurons were NF200-positive. Intra-sciatic nerve injection of CTB labeled over 30% of the VR1-positive neurons in the L5 DRG. Size frequency distribution analysis revealed that these VR1 and NF200, or VR1 and CTB, double-labeled neurons were predominantly small and medium sized. These results suggest that capsaicin receptors are likely to be expressed by Aδ-fiber neurons as well as C-fiber neurons.",

keywords = "Aδ-fibers, C-fibers, Capsaicin, Cholera toxin, Dorsal root ganglia, Neurofilament, VR1 receptor",

author = "Ma, {Qing Ping}",

year = "2002",

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doi = "10.1016/S0304-3940(01)02537-X",

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T1 - Expression of capsaicin receptor (VR1) by myelinated primary afferent neurons in rats

AU - Ma, Qing Ping

PY - 2002/2/15

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N2 - The expression of capsaicin (VR1) receptor by A-fiber primary afferent neurons has been investigated by double immunohistochemical staining with VR1 and 200 kD neurofilament (NF200; an A-fiber marker) antibodies, and by VR1 immunohistochemical staining in combination with cholera toxin B subunit (CTB; also an A-fiber marker) retrograde tracing. Approximately 30% of the VR1-positive dorsal root ganglion (DRG) neurons were NF200-positive. Intra-sciatic nerve injection of CTB labeled over 30% of the VR1-positive neurons in the L5 DRG. Size frequency distribution analysis revealed that these VR1 and NF200, or VR1 and CTB, double-labeled neurons were predominantly small and medium sized. These results suggest that capsaicin receptors are likely to be expressed by Aδ-fiber neurons as well as C-fiber neurons.

AB - The expression of capsaicin (VR1) receptor by A-fiber primary afferent neurons has been investigated by double immunohistochemical staining with VR1 and 200 kD neurofilament (NF200; an A-fiber marker) antibodies, and by VR1 immunohistochemical staining in combination with cholera toxin B subunit (CTB; also an A-fiber marker) retrograde tracing. Approximately 30% of the VR1-positive dorsal root ganglion (DRG) neurons were NF200-positive. Intra-sciatic nerve injection of CTB labeled over 30% of the VR1-positive neurons in the L5 DRG. Size frequency distribution analysis revealed that these VR1 and NF200, or VR1 and CTB, double-labeled neurons were predominantly small and medium sized. These results suggest that capsaicin receptors are likely to be expressed by Aδ-fiber neurons as well as C-fiber neurons.

KW - Aδ-fibers

KW - C-fibers

KW - Capsaicin

KW - Cholera toxin

KW - Dorsal root ganglia

KW - Neurofilament

KW - VR1 receptor

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AN - SCOPUS:0037083139

SN - 0304-3940

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JF - Neuroscience Letters

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ER -

Expression of capsaicin receptor (VR1) by myelinated primary afferent neurons in rats

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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