Differential microRNA expression and identification of putative miRNA targets and pathways in head and neck cancers

Nurul Syakima Ab Mutalib, Cheah Yoke-Kqueen, Sabariah Abdul Rahman, Shiran Mohd Sidik, Avatar Singh Mohan Singh, Lee Learn-Han

Research output: Journal PublicationArticlepeer-review

57 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that involved in various cancer-related cellular processes. Diverse studies on expression profiling of miRNAs have been performed and the data showed that some miRNAs are up-regulated or down-regulated in cancer. Until now, there are no data published on the miRNA expression in head and neck cancers from Malaysia. Hence, this study aimed to investigate potentially crucial miRNAs in head and neck cancer patients from Malaysian populations. A global miRNA profiling was performed on 12 samples of head and neck cancer tissue using microarray analysis followed by validation using real-time RT-PCR Microarray analysis identified 10 miRNAs that could distinguish malignant head and neck cancer lesions from normal tissues; 7 miRNAs (hsa-miR-181a-2*, hsa-miR-29b-1*, hsa-miR-181a, hsa-miR-181b, hsa-miR-744, hsa-miR-1271 and hsa-miR-221*) were up-regulated while 3 miRNAs (hsa-miR-141, hsa-miR-95 and hsa-miR-101) were down-regulated. These miRNAs may contribute in a simple profiling strategy to identify individuals at higher risk of developing head and neck cancers, thus helping in the elucidation of the molecular mechanisms involved in head and neck cancer pathogenesis.

Original languageEnglish
Pages (from-to)327-336
Number of pages10
JournalInternational Journal of Molecular Medicine
Volume28
Issue number3
DOIs
Publication statusPublished - Sept 2011
Externally publishedYes

Keywords

  • Head and neck cancers
  • In silico analysis
  • Microarray
  • Quantitative reverse transcription-PCR
  • microRNA

ASJC Scopus subject areas

  • Genetics

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