Development of novel FRET aptasensor based on the quenching ability of iron oxide-gold nanostars for the detection of aflatoxin M1

Faizan Ul Haq, Aasma Batool, Sobia Niazi, Imran Mahmood Khan, Ali Raza, Ding Ning, Yin Zhang, Zhouping Wang

Research output: Journal PublicationArticlepeer-review

1 Citation (Scopus)

Abstract

Detecting Aflatoxin M1 (AFM1) in food products is crucial due to its high toxicity and health risks. This study introduced a fluorescence donor material using Rhodamine-B-Isothiocyanate (RBITC)-doped silica nanoparticles (RDSN) combined with iron oxide‑gold nanostars (IOGNS) as a quencher. The composite aptasensor (RDSN/IOGNS) served as a Förster Resonance Energy Transfer (FRET) nanoprobe for sensitive and selective AFM1 detection. The fluorescence of aptamer-conjugated RDSN (apt-RDSN) was effectively quenched by complementary DNA-conjugated IOGNS (cDNA-IOGNS). Upon AFM1 introduction, apt-RDSN dissociated from the IOGNS surface, restoring the fluorescence signal. The fluorescence intensity correlated linearly with AFM1 concentration, achieving a detection limit of 0.15 ng/mL. Compared to conventional enzyme-linked immunosorbent assay (ELISA), this FRET aptasensor showed excellent recovery rate and relative standard deviation (RSD) in milk samples, highlighting its potential for practical AFM1 detection applications.

Original languageEnglish
Article number141575
JournalFood Chemistry
Volume464
DOIs
Publication statusPublished - 1 Feb 2025

Keywords

  • AFM1
  • Food safety
  • Nano sensor
  • RBITC doped silica nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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