Abstract
Detecting Aflatoxin M1 (AFM1) in food products is crucial due to its high toxicity and health risks. This study introduced a fluorescence donor material using Rhodamine-B-Isothiocyanate (RBITC)-doped silica nanoparticles (RDSN) combined with iron oxide‑gold nanostars (IOGNS) as a quencher. The composite aptasensor (RDSN/IOGNS) served as a Förster Resonance Energy Transfer (FRET) nanoprobe for sensitive and selective AFM1 detection. The fluorescence of aptamer-conjugated RDSN (apt-RDSN) was effectively quenched by complementary DNA-conjugated IOGNS (cDNA-IOGNS). Upon AFM1 introduction, apt-RDSN dissociated from the IOGNS surface, restoring the fluorescence signal. The fluorescence intensity correlated linearly with AFM1 concentration, achieving a detection limit of 0.15 ng/mL. Compared to conventional enzyme-linked immunosorbent assay (ELISA), this FRET aptasensor showed excellent recovery rate and relative standard deviation (RSD) in milk samples, highlighting its potential for practical AFM1 detection applications.
Original language | English |
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Article number | 141575 |
Journal | Food Chemistry |
Volume | 464 |
DOIs | |
Publication status | Published - 1 Feb 2025 |
Keywords
- AFM1
- Food safety
- Nano sensor
- RBITC doped silica nanoparticles
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science