Construction of cell-compatible layer and culture of human umbilical vascular endothelial cells on porous polystyrene membranes

Changyou Gao, An Li, Xiaosu Yi, Jiacong Shen

Research output: Journal PublicationArticlepeer-review

15 Citations (Scopus)

Abstract

Porous polystyrene (PS) membranes with pore sizes ranged from nanometer scale to several decades' micrometers were fabricated by the thermally induced phase-separation technique, and were characterized by scanning electron microscopy (SEM). Oxidation of PS membranes by concentrated sulfuric acid induced a decrease of the carbon amount on the membrane surfaces accompanying with an increase of the oxygen amount. The hydrophilic surfaces thus constructed proved quite effective to promote endothelial cell adhesion and growth. After culturing for 4 days, a confluent endothelial cells (ECs) layer was observed on nonporous PS membrane treated previously with sulfuric acid for 15 min at 28.5°C. The observation under SEM showed that ECs on the PS membrane surfaces at the fourth day were inversely proportional to the pore size: the smaller the pore size, the larger the amount of ECs on the membrane surfaces. It is supposed that the existence of pores on the membrane surface might generate some disadvantageous resistance to the spreading of cells.

Original languageEnglish
Pages (from-to)3523-3529
Number of pages7
JournalJournal of Applied Polymer Science
Volume81
Issue number14
DOIs
Publication statusPublished - 29 Sep 2001
Externally publishedYes

Keywords

  • Endothelial cell
  • Porous polystyrene membrane
  • Thermally induced phase separation
  • Tissue engineering

ASJC Scopus subject areas

  • Chemistry (all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

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