Biomimetic self-assembly of lipase-zeolitic imidazolate frameworks with enhanced biosensing of protox inhibiting herbicides

Chuanchuan Cheng, Cai Shen, Oi Ming Lai, Chin Ping Tan, Ling Zhi Cheong

Research output: Journal PublicationArticlepeer-review

1 Citation (Scopus)

Abstract

Protox inhibiting herbicides such as nitrofen have detrimental effects on the environment and human health. The current work aims to fabricate a Candida rugosa lipase (CRL)-based electrochemical sensor for rapid and sensitive detection of protox inhibiting herbicides (nitrofen). We proposed the use of poly(vinylpyrrolidone) (PVP) and amino-acids to promote accumulation of Zn2+ ions at the surfaces of Candida rugosa lipase (CRL) and subsequently induce self-assembly of a CRL-zeolitic imidazolate framework (ZIF) structure. This process can be easily and rapidly achieved via a one-pot facile self-assembly method. Steady-state fluorescence spectroscopy indicated that CRL has undergone a conformational change following encapsulation within the ZIF structure. This conformational change is beneficial as the prepared PVP/Glu/CRL@ZIF-8 exhibited enhanced catalytic activity (207% of native CRL), and higher substrate affinity (lower Km than native CRL) and showed high stability under harsh denaturing conditions. PVP/Glu/CRL@ZIF-8 was finally used for electrochemical biosensing of nitrofen. The fabricated biosensor has a wide linear detection range (0-100 μM), a lower limit of detection and a good recovery rate.

Original languageEnglish
Pages (from-to)4974-4984
Number of pages11
JournalAnalytical Methods
Volume13
Issue number42
DOIs
Publication statusPublished - 14 Nov 2021
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering (all)
  • Engineering (all)

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