Basal expression of bradykinin B1 receptor in the spinal cord in humans and rats

Q. P. Ma, R. Heavens

Research output: Journal PublicationArticlepeer-review

36 Citations (Scopus)

Abstract

The bradykinin B1 receptor has been considered as a receptor induced by tissue injury and inflammation mainly in the peripheral tissues. In the present study, we have investigated whether there is a basal expression in the spinal cord by both reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining methods. Southern blotting of the DNA reverse-transcribed from human and rat spinal cord mRNA and amplified by polymerase chain reaction (PCR) showed a substantial basal B1 receptor expression in both human and rat spinal cord. Immunohistochemical staining demonstrated B1-positive neurons in the spinal cord dorsal horn, suggesting that the B1 receptor is constitutively expressed by spinal neurons.

Original languageEnglish
Pages (from-to)2311-2314
Number of pages4
JournalNeuroReport
Volume12
Issue number11
DOIs
Publication statusPublished - 8 Aug 2001
Externally publishedYes

Keywords

  • B receptor
  • Bradykinin
  • Dorsal horn
  • Immunohistochernistry
  • Polymerase chain reaction (PCR)

ASJC Scopus subject areas

  • Neuroscience (all)

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