Aptamers selected and identified as a colorimetric dual-mode sensing platform for selective and sensitive detection of capsaicin

Mingwei Qin, Imran Mahmood Khan, Ning Ding, Yuhan Sun, Shuo Qi, Xiaoze Dong, Sobia Niazi, Yin Zhang, Zhouping Wang

Research output: Journal PublicationArticlepeer-review

2 Citations (Scopus)

Abstract

Capsaicin (CAP), a prominent indicator, has been identified for detecting the gutter oil in adulterated edible oils. In view of the limited availability of efficient and reliable methods for detecting gutter oil, the present study employed the Capture-SELEX technology, which utilizes a high-throughput sequencing (HTS) platform, to facilitate the precise tracking of screening rounds and facilitate the isolation of aptamers with specific affinity for CAP. Isothermal titration calorimetry (ITC) determined the highest affinity and Kd value of 15.5 ± 4.69 nM for aptamer Cap-1. A colorimetric dual-mode aptasensor was designed for CAP detection based on graphene oxide (GO)@gold nanoparticles (AuNPs) composite. A high-sensitivity assay can be achieved using a Synergy™ H1 multimode zymography instrument and a smartphone. Under optimal conditions, the colorimetric dual-mode aptasensor has a detection range of 0.5 ∼ 120 ng mL−1 and a low limit of detection (LOD) of 0.14 ng mL−1. The quantitative recovery of the sensor for CAP in edible oil was 86.25∼98.12 %. Compared with the conventional single-mode aptasensor, the dual-mode aptasensor has independent signal conversion, which provides suitable detection solutions for different scenarios and improves detection accuracy and reliability.

Original languageEnglish
Article number134638
JournalSensors and Actuators, B: Chemical
Volume396
DOIs
Publication statusPublished - 1 Dec 2023
Externally publishedYes

Keywords

  • Aptamers
  • Capsaicin
  • Capture-SELEX
  • Dual-mode

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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