Antimicrobial framework nucleic acid-based DNAzyme cluster with high catalytic efficiency

Noshin Afshan, Ruba Tariq, Iqra Riaz, Abdul Manan, Azhar Iqbal, Muhammad Ejaz, Amir Sohail, Alina Bari, Sajid Mahmood, Shahid Iqbal, Khalid M. Alotaibi, Matar Alshalwi

Research output: Journal PublicationArticlepeer-review

Abstract

BACKGROUND: Hydroxyl radical-mediated materials primarily liberate more reactive and acutely lethal hydroxyl radical (OH) and act as potent bactericidal antibiotics, for example H2O2. Hydroxyl radical possess higher tendency than that of H2O2 to attack various biological molecules such as DNA, proteins and iron–sulfur clusters, and impair their proper functioning, actively leading to strongly potent bactericidal effect. To acquire the desired antimicrobial effect, high concentration of H2O2 is required that has found medically harmful to healthy tissues of humans. RESULTS: We herein report framework nucleic acid-regulated DNAzyme cluster (FDC) – that is, peroxidase-like hemin-bound G-quadruplex (G4/H) DNAzyme – to amplify the catalytic reduction potential of G4/H complex, leading to high conversion rate of H2O2 to more reactive hydroxyl radical that potentially shows the same antibacterial efficiency at lower and safer H2O2 concentration. Specifically, we have grafted multiple copies of DNAzymes outside framework nucleic acid (FNA) to successively achieve 3–9 orders of magnitude enhancement in catalytic activity and antibacterial efficiency of FDC. CONCLUSION: Our designed FDC has successfully alleviated H2O2 toxicity and increased its efficiency as antibacterial material, as FDC amplified the catalytic reduction potential of G4/H DNAzyme, leading to high conversion rate of H2O2 to more reactive OH that potentially shows the same antibacterial efficiency at lower and safer H2O2 concentration.

Original languageEnglish
Pages (from-to)2027-2034
Number of pages8
JournalJournal of Chemical Technology and Biotechnology
Volume99
Issue number9
DOIs
Publication statusPublished - Sept 2024

Keywords

  • DNAzyme
  • G-quadruplex
  • antibacterial
  • binding constant
  • framework nucleic acid
  • hydroxyl radical
  • nanotechnology
  • polyacrylamide gel electrophoresis (PAGE)
  • self-assembly

ASJC Scopus subject areas

  • Biotechnology
  • General Chemical Engineering
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

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