Analysis of Salmonella Agona and Salmonella Weltevreden in Malaysia by PCR fingerprinting and antibiotic resistance profiling

Lee Learn-Han, Cheah Yoke-Kqueen, Noorzaleha Awang Salleh, Sabrina Sukardi, Sim Jiun-Horng, Khoo Chai-Hoon, Son Radu

Research output: Journal PublicationArticlepeer-review

30 Citations (Scopus)

Abstract

Forty-eight strains of Salmonella enterica subsp. enterica serovar Agona and 33 strains of Salmonella enterica subsp. enterica serovar Weltevreden were characterized by random amplified polymorphic DNA (RAPD) fingerprinting using 3 different arbitrary primer, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) and antimicrobial susceptibility testing. By using RAPD, 81 strains (44 strains of S. Agona and 33 strains of S. Weltevreden) can be clustered into 14 groups and 6 single isolates whereas ERIC-PCR produced 7 clusters and 3 single isolates. Thirteen antimicrobial agents were used and all the isolates were resistant to erythromycin and showed Multiple Antimicrobial Resistance indexes, ranging from 0.08 to 0.62. Poultry still remain as the common reservoir for multi-drug-resistant Salmonella. On the other hand, vegetables contaminated with S. Weltevreden showed a gain in antimicrobial resistance. Besides that, consistent antibiograms were observed from S. Weltevreden isolated at Kajang wet market on 2000/08/02.

Original languageEnglish
Pages (from-to)377-387
Number of pages11
JournalAntonie van Leeuwenhoek
Volume94
Issue number3
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Composite analysis
  • ERIC-PCR
  • Multiple antimicrobial resistance
  • RAPD
  • Salmonella Agona
  • Salmonella Weltevreden

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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