TY - JOUR
T1 - Alternansucrase as a key enabling tool of biotransformation from molecular features to applications
T2 - A review
AU - Chen, Gang
AU - Khan, Imran Mahmood
AU - Zhang, Tao
AU - Campanella, Osvaldo H.
AU - Miao, Ming
N1 - Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/11
Y1 - 2024/11
N2 - Alternansucrase (ASR), classified in GH70, produces unique α-glucans with alternating α-1,3 and α-1,6 glycosidic linkages in the backbone chain from renewable sucrose which is easily obtained from nature with low cost. ASR has synthesized many products with valuable functionalities that hold enormous commercial interest and promising applications. The influence of biocatalysis and fermentation parameters on the yields, and properties of products are critical for the propositions made to promote the enzyme application. Investigations on ASR have been compiled in the review to provide information on the enzyme, products and parameters. This review summarizes studies on the characteristics, conversion mechanism, products, and beneficial applications of ASR and exhibits structure-based technologies to improve enzyme activity, specificity, and thermostability for industrial applications. Finally, prospects for further development are also proposed for various ASR applications in food and other fields.
AB - Alternansucrase (ASR), classified in GH70, produces unique α-glucans with alternating α-1,3 and α-1,6 glycosidic linkages in the backbone chain from renewable sucrose which is easily obtained from nature with low cost. ASR has synthesized many products with valuable functionalities that hold enormous commercial interest and promising applications. The influence of biocatalysis and fermentation parameters on the yields, and properties of products are critical for the propositions made to promote the enzyme application. Investigations on ASR have been compiled in the review to provide information on the enzyme, products and parameters. This review summarizes studies on the characteristics, conversion mechanism, products, and beneficial applications of ASR and exhibits structure-based technologies to improve enzyme activity, specificity, and thermostability for industrial applications. Finally, prospects for further development are also proposed for various ASR applications in food and other fields.
KW - Alternansucrase
KW - Applications
KW - Molecular features
UR - http://www.scopus.com/inward/record.url?scp=85202757059&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2024.135096
DO - 10.1016/j.ijbiomac.2024.135096
M3 - Review article
C2 - 39214198
AN - SCOPUS:85202757059
SN - 0141-8130
VL - 279
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 135096
ER -