A proof of concept: Detection of avian influenza H5 gene by a graphene-enhanced electrochemical genosensor

Sze Shin Low, Joanna Su Yuin Chia, Michelle T.T. Tan, Hwei San Loh, Poi Sim Khiew, Ajit Singh, Wee Siong Chiu

Research output: Journal PublicationArticlepeer-review

13 Citations (Scopus)

Abstract

The highly pathogenic avian influenza (HPAI) virus subtype H5N1 has been found to be the most virulent and deadliest virus strain, with capability of interspecies transmission to human infection. Most human HPAI H5N1 cases were diagnosed late in their illnesses for medical care, resulting in severe complications that led to death. In this study, a novel graphene-enhanced electrochemical DNA biosensor had been fabricated for the detection of polymerase chain reaction (PCR) amplicon derived from the haemagglutinin (H5) gene of the HPAI. The graphene-enhanced DNA biosensor showed excellent linear correlation between PCR amplicon concentration and amperometric signal with a correlation coefficient, r 2 of 0.9987. The amperometric response of the proposed biosensor was compared with conventional gel electrophoresis while the feasibility of the proposed sandwich sensing platform was verified via dot blot assay. The results obtained indicate that the electrochemical DNA biosensing assay is significantly more sensitive (P <0,05) and time efficient. This work serves as a proof of concept in hopes for further development of the graphene enhanced electrochemical DNA biosensor into a portable, on-site screening platform for point-of-care detection of various pathogens.

Original languageEnglish
Pages (from-to)2438-2446
Number of pages9
JournalJournal of Nanoscience and Nanotechnology
Volume16
Issue number3
DOIs
Publication statusPublished - Mar 2016
Externally publishedYes

Keywords

  • Avian influenza virus
  • Electrochemical genosensor
  • Graphene
  • PCR amplicons
  • Screen-printed carbon electrode

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry (all)
  • Biomedical Engineering
  • Materials Science (all)
  • Condensed Matter Physics

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