A novel bioassay based on aptamer-functionalized magnetic nanoparticle for the detection of zearalenone using time resolved-fluorescence NaYF4: Ce/Tb nanoparticles as signal probe

Sobia Niazi, Xiaole Wang, Imran Pasha, Imran Mahmood Khan, Sen Zhao, Muhammad Shoaib, Shijia Wu, Zhouping Wang

Research output: Journal PublicationArticlepeer-review

62 Citations (Scopus)

Abstract

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by fungi on stored grains. The earlier detection methods used for ZEN rely on expensive equipment, time-consuming sample preparation and temperature sensitive antibodies. The current work, proposed a novel strategy based on ZEN aptamer labeled with amine-functionalized magnetic nanoparticle (MNPs) as a capture probe and time-resolved fluorescence (TRFL) nanoparticles labeled with complementary DNA (cDNA) as a signal probe. Under the optimized conditions, TRFL intensity at 544 nm was used to measure ZEN (R2 = 0.9920) in the range of 0.001–10 ng mL−1 and limits of detection (LOD) for proposed method was 0.21 pg mL−1. The specificity of bioassay was also determined by using other mycotoxins (OTA, AFB2, DON and Patulin) and results showed that the aptamer are specific to recognize only ZEN. The analytical applications of the present bioassay in maize and wheat samples were also examined and results were compared with existing methods. Based on these findings, it is suggested to use current rapid and simple bioassay for the determination of ZEN in food and agricultural products.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalTalanta
Volume186
DOIs
Publication statusPublished - 15 Aug 2018
Externally publishedYes

Keywords

  • Aptamer
  • Bioassay
  • Magnetic nanoparticles
  • Time-resolved fluorescence nanoparticles
  • Zearalenone

ASJC Scopus subject areas

  • Analytical Chemistry

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