A modified Langmuir model for the prediction of the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents

Q. Lan, A. S. Bassi, J. X. Zhu, A. Margaritis

Research output: Journal PublicationArticlepeer-review

118 Citations (Scopus)

Abstract

An empirical model modified from the Langmuir isotherm model to account for the effects of ionic strength on the equilibrium characteristics of protein adsorption onto ion exchange/affinity adsorbents has been proposed and tested against experimental and literature data. The equilibrium isotherms for BSA adsorption onto a polystyrenic anion exchanger, Diaion HPA25, were established for five different NaCl concentrations at 25°C, pH 7.0. The apparent Langmuir parameters in the new model (q′m and K′d), which replace the Langmuir parameters (qm and Kd) in the original Langmuir model, were determined by non-linear curve fitting. The proposed model has been shown to be applicable to various protein/adsorbent systems.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalChemical Engineering Journal
Volume81
Issue number1-3
DOIs
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Keywords

  • Ion exchange
  • Ionic strength
  • Langmuir isotherm
  • Modeling
  • Protein adsorption

ASJC Scopus subject areas

  • Chemistry (all)
  • Environmental Chemistry
  • Chemical Engineering (all)
  • Industrial and Manufacturing Engineering

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