Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

Bao Kai Ma; Mian Li; Ling Zhi Cheong; Xin Chu Weng; Cai Shen; Qing Huang

doi:10.15541/jim20190139

Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H₂O₂

Bao Kai Ma, Mian Li, Ling Zhi Cheong, Xin Chu Weng, Cai Shen, Qing Huang

Research output: Journal Publication › Article › peer-review

26 Citations (Scopus)

Abstract

Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H₂O₂) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H₂O₂. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol∙L^-1, a limit of detection of 0.74 μmol∙L^-1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H₂O₂ in both solid and liquid food.

Translated title of the contribution	酶-二维MXene复合材料的制备及其电化学检测H₂O₂的应用
Original language	English
Pages (from-to)	131-138
Number of pages	8
Journal	Wuji Cailiao Xuebao/Journal of Inorganic Materials
Volume	35
Issue number	1
DOIs	https://doi.org/10.15541/jim20190139
Publication status	Published - 1 Jan 2020
Externally published	Yes

Keywords

Biosensor
Horse radish peroxidase
Hydrogen peroxide
MXene nanosheets

ASJC Scopus subject areas

General Materials Science
Inorganic Chemistry

Access to Document

10.15541/jim20190139

Cite this

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title = "Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2",

abstract = "Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol∙L-1, a limit of detection of 0.74 μmol∙L-1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.",

keywords = "Biosensor, Horse radish peroxidase, Hydrogen peroxide, MXene nanosheets",

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T1 - Enzyme-MXene Nanosheets

T2 - Fabrication and Application in Electrochemical Detection of H2O2

AU - Ma, Bao Kai

AU - Li, Mian

AU - Cheong, Ling Zhi

AU - Weng, Xin Chu

AU - Shen, Cai

AU - Huang, Qing

PY - 2020/1/1

Y1 - 2020/1/1

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AB - Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol∙L-1, a limit of detection of 0.74 μmol∙L-1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.

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KW - Horse radish peroxidase

KW - Hydrogen peroxide

KW - MXene nanosheets

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