辣根过氧化物酶-金属有机骨架 纳米纤维复合物生物传感器的制备及其 用于食品中过氧化氢残留检测的研究

Translated title of the contribution: Fabrication of Horseradish Peroxidase-metal Organic Nanofibers Biosensor and Its Application in Detection of Hydrogen Peroxide Residues in Food

Chuan Chuan Cheng, Bao Kai Ma, Yong Fa Cheng, Cai Shen, Xin Chu Weng, Ling Zhi Zhang

Research output: Journal PublicationArticlepeer-review

3 Citations (Scopus)

Abstract

Metal-organic framework nanofibers were used as horseradish peroxidase immobilization materials, chitosan was used as a film-forming agent to prepare an electrochemical biosensor ( HRP- MONFs/Chit/GCE ) for the detection of hydrogen peroxide residues in foods by drop coating. Drop cast method was used to fabricate the electrochemical biosensor. The fabricated biosensor was evaluated in terms of electrochemical properties including electrochemical impedance (EIS) and cyclic voltammetry(CV), and the preparation method and detection conditions were optimized. Results showed that, HRP-MONFs/ Chit/GCE exhibited a good linear relationship(R2 =0.999) in the range of hydrogen peroxide concentration of 12.5∼675 μmol/L, and the minimum detection limit was 0.97 mol/L.HRP-MONFs/Chit/GCE had good storage stability, which was able to retain 94% of its orginal response during the 24 days storage test at 4 °C.The fabricated biosensorwas successfully employed for detection of trace level of hydrogen peroxide in both solid and liquid food.

Translated title of the contributionFabrication of Horseradish Peroxidase-metal Organic Nanofibers Biosensor and Its Application in Detection of Hydrogen Peroxide Residues in Food
Original languageChinese (Traditional)
Pages (from-to)199-204 and 212
JournalScience and Technology of Food Industry
Volume40
Issue number24
DOIs
Publication statusPublished - 2019
Externally publishedYes

ASJC Scopus subject areas

  • Food Science

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