TY - JOUR
T1 - Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis
T2 - A systematically modeled approach
AU - Mohsin, Ali
AU - Sun, Jingyun
AU - Khan, Imran Mahmood
AU - Hang, Haifeng
AU - Tariq, Muhammad
AU - Tian, Xiwei
AU - Ahmed, Waqas
AU - Niazi, Sobia
AU - Zhuang, Yingping
AU - Chu, Ju
AU - Mohsin, Muhammad Zubair
AU - Salim-ur-Rehman,
AU - Guo, Meijin
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/2/1
Y1 - 2019/2/1
N2 - This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60–120 °C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95–98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate (μm = 0.233/h), highest curdlan production (Pm = 23.24 g/L) and growth associated rate constant (α = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.
AB - This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60–120 °C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95–98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate (μm = 0.233/h), highest curdlan production (Pm = 23.24 g/L) and growth associated rate constant (α = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.
KW - Batch culture fermentation
KW - Curdlan
KW - Kinetics
KW - Orange peels
KW - Sustainable biosynthesis
UR - https://www.scopus.com/pages/publications/85055970719
U2 - 10.1016/j.carbpol.2018.10.047
DO - 10.1016/j.carbpol.2018.10.047
M3 - Article
C2 - 30446148
AN - SCOPUS:85055970719
SN - 0144-8617
VL - 205
SP - 626
EP - 635
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -