Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil

Learn Han Lee; Adzzie Shazleen Azman; Nurullhudda Zainal; Wai Fong Yin; Nurul Syakima Ab Mutalib; Kok Gan Chan

doi:10.1099/ijs.0.000053

Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil

Learn Han Lee
, Adzzie Shazleen Azman
, Nurullhudda Zainal
, Wai Fong Yin
, Nurul Syakima Ab Mutalib
, Kok Gan Chan

Research output: Journal Publication › Article › peer-review

26 Citations (Scopus)

Abstract

Strain MUSC 117^Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117^Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127^T(98.0%), Sinomonas albida LC13^T(97.9%) and Sinomonas soli CW 59T (97.8%), and lower (<97.6%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27%) between strain MUSC 117^Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3_α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0%) of the cell membrane were anteiso-C_{15: 0}(39.4%), C_{18: 1}ω7c (17.7%), anteiso-C_{17: 0}(17.2%) and iso-C_{16: 0}(11.4%). The predominant respiratory quinones detected were MK-9(H₂) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117^Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117^Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117^T(5DSM 29362^T=MCCC 1K00410^T=NBRC 110653^T).

Original language	English
Pages (from-to)	996-1002
Number of pages	7
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	65
Issue number	3
DOIs	https://doi.org/10.1099/ijs.0.000053
Publication status	Published - 2015
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/ijs.0.000053

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@article{36ce36aa97fc4aaca22e2dd290908d0c,

title = "Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil",

abstract = "Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0\%), Sinomonas albida LC13T(97.9\%) and Sinomonas soli CW 59T (97.8\%), and lower (<97.6\%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27\%) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0\%) of the cell membrane were anteiso-C15: 0(39.4\%), C18: 1ω7c (17.7\%), anteiso-C17: 0(17.2\%) and iso-C16: 0(11.4\%). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol\%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).",

author = "Lee, \{Learn Han\} and Azman, \{Adzzie Shazleen\} and Nurullhudda Zainal and Yin, \{Wai Fong\} and \{Ab Mutalib\}, \{Nurul Syakima\} and Chan, \{Kok Gan\}",

note = "Publisher Copyright: {\textcopyright} 2015 IUMS.",

year = "2015",

doi = "10.1099/ijs.0.000053",

language = "English",

volume = "65",

pages = "996--1002",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Microbiology Society",

number = "3",

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TY - JOUR

T1 - Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil

AU - Lee, Learn Han

AU - Azman, Adzzie Shazleen

AU - Zainal, Nurullhudda

AU - Yin, Wai Fong

AU - Ab Mutalib, Nurul Syakima

AU - Chan, Kok Gan

PY - 2015

Y1 - 2015

N2 - Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0%), Sinomonas albida LC13T(97.9%) and Sinomonas soli CW 59T (97.8%), and lower (<97.6%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27%) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0%) of the cell membrane were anteiso-C15: 0(39.4%), C18: 1ω7c (17.7%), anteiso-C17: 0(17.2%) and iso-C16: 0(11.4%). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).

AB - Strain MUSC 117Twas isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod–coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117Texhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T(98.0%), Sinomonas albida LC13T(97.9%) and Sinomonas soli CW 59T (97.8%), and lower (<97.6%) sequence similarity to other species of the genus Sinomonas. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 27%) between strain MUSC 117Tand closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (.10.0%) of the cell membrane were anteiso-C15: 0(39.4%), C18: 1ω7c (17.7%), anteiso-C17: 0(17.2%) and iso-C16: 0(11.4%). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117Trepresented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117Trepresents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117T(5DSM 29362T=MCCC 1K00410T=NBRC 110653T).

UR - https://www.scopus.com/pages/publications/84925296589

U2 - 10.1099/ijs.0.000053

DO - 10.1099/ijs.0.000053

M3 - Article

C2 - 25563924

AN - SCOPUS:84925296589

SN - 1466-5026

VL - 65

SP - 996

EP - 1002

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 3

ER -

Sinomonas humi sp. nov., an amylolytic actinobacterium isolated from mangrove forest soil

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