Ribonucleic-Acid protein interaction prediction based on deep learning: A comprehensive survey

The interaction between Ribonucleic Acids (RNAs) and proteins, also called RNA Protein Interaction (RPI), governs biological processes, including gene regulation and disease pathogenesis. This comprehensive survey examines Artificial Intelligence (AI) applications in Deep Learning-based RPI Prediction (DL-based RPIP) through eight Research Questions (RQs), analyzing 179 studies (2014–2023). The key findings include: sustained technical evolution through embryonic (2014–2017), accelerated (2018–2022), and expansion phases (2023) (RQ1); hybrid models integrating Graph Neural Networks (GNNs) (for topological interface modeling) and Transformers (for long-range dependencies) achieve state-of-the-art performance (RQ4); pretrained language models enhance small-sample learning, but the cross-species generalization declines sharply with evolutionary distance (RQ5). Critical challenges persist, including data heterogeneity across databases, the scarcity of standardized benchmarks (RQ2), and balancing the trade-off between feature encoding and information preservation (RQ3). Future advancements require biologically informed DL architectures, multi-feature fusion, and rigorous cross-validation to bridge the generalization-interpretability gap (RQ8): This would accelerate the clinical translation of predictive tools (RQ6/RQ7). As the first comprehensive analysis spanning feature encoding, modeling, evaluation, applications, and tools, this work fills a critical gap in the DL-based RPIP literature.